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2cg4

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[[Image:2cg4.gif|left|200px]]<br /><applet load="2cg4" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2cg4, resolution 2.40&Aring;" />
 
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'''STRUCTURE OF E.COLI ASNC'''<br />
 
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==Overview==
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==Structure of E.coli AsnC==
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The Lrp/AsnC family of transcriptional regulatory proteins is found in, both archaea and bacteria. Members of the family influence cellular, metabolism in both a global (Lrp) and specific (AsnC) manner, often in, response to exogenous amino acid effectors. In the present study we have, determined both the first bacterial and the highest resolution structures, for members of the family. Escherichia coli AsnC is a specific gene, regulator whose activity is triggered by asparagine binding. Bacillus, subtilis LrpC is a global regulator involved in chromosome condensation., Our AsnC-asparagine structure is the first for a regulator-effector, complex and is revealed as an octameric disc. Key ligand recognition, residues are identified together with a route for ligand access. The LrpC, structure reveals a stable octamer supportive of a topological role in, dynamic DNA packaging. The structures yield significant clues to the, functionality of Lrp/AsnC-type regulators with respect to ligand binding, and oligomerization states as well as to their role in specific and global, DNA regulation.
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<StructureSection load='2cg4' size='340' side='right'caption='[[2cg4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2cg4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2CG4 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASN:ASPARAGINE'>ASN</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2cg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2cg4 OCA], [https://pdbe.org/2cg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2cg4 RCSB], [https://www.ebi.ac.uk/pdbsum/2cg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2cg4 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ASNC_ECOLI ASNC_ECOLI] Activator of asnA transcription; autogenous regulator of its own transcription; and repressor of the expression of gidA at a post-transcriptional level.<ref>PMID:3909107</ref> <ref>PMID:2864330</ref> <ref>PMID:2836709</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/2cg4_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2cg4 ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2CG4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=MG:'>MG</scene> and <scene name='pdbligand=ASN:'>ASN</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CG4 OCA].
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*[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural insight into gene transcriptional regulation and effector binding by the Lrp/AsnC family., Thaw P, Sedelnikova SE, Muranova T, Wiese S, Ayora S, Alonso JC, Brinkman AB, Akerboom J, van der Oost J, Rafferty JB, Nucleic Acids Res. 2006 Mar 9;34(5):1439-49. Print 2006. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16528101 16528101]
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Akerboom, J.]]
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[[Category: Akerboom J]]
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[[Category: Alonso, J.C.]]
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[[Category: Alonso JC]]
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[[Category: Ayora, S.]]
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[[Category: Ayora S]]
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[[Category: Brinkman, A.B.]]
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[[Category: Brinkman AB]]
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[[Category: Muranova, T.]]
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[[Category: Muranova T]]
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[[Category: Oost, J.Van.Der.]]
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[[Category: Rafferty JB]]
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[[Category: Rafferty, J.B.]]
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[[Category: Sedelnikova SE]]
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[[Category: Sedelnikova, S.E.]]
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[[Category: Thaw P]]
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[[Category: Thaw, P.]]
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[[Category: Wiese S]]
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[[Category: Wiese, S.]]
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[[Category: Van der Oost J]]
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[[Category: ASN]]
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[[Category: MG]]
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[[Category: asnc]]
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[[Category: dna binding]]
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[[Category: dna-binding]]
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[[Category: ffrp]]
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[[Category: lrp family]]
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[[Category: transcription]]
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[[Category: transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Feb 3 10:34:58 2008''
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Current revision

Structure of E.coli AsnC

PDB ID 2cg4

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