2d1u

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[[Image:2d1u.gif|left|200px]]
 
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==Solution structure of the periplasmic signaling domain of FecA from Escherichia coli==
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The line below this paragraph, containing "STRUCTURE_2d1u", creates the "Structure Box" on the page.
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<StructureSection load='2d1u' size='340' side='right'caption='[[2d1u]]' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2d1u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2D1U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2d1u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2d1u OCA], [https://pdbe.org/2d1u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2d1u RCSB], [https://www.ebi.ac.uk/pdbsum/2d1u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2d1u ProSAT]</span></td></tr>
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{{STRUCTURE_2d1u| PDB=2d1u | SCENE= }}
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</table>
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== Function ==
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'''Solution strcuture of the periplasmic signaling domain of FecA from Escherichia coli'''
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[https://www.uniprot.org/uniprot/FECA_ECOLI FECA_ECOLI] FecA is the outer membrane receptor protein in the Fe(3+) dicitrate transport system.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Gram-negative bacteria possess outer membrane receptors that utilize energy provided by the TonB system to take up iron. Several of these receptors participate in extracytoplasmic factor (ECF) signalling through an N-terminal signalling domain that interacts with a periplasmic transmembrane anti-sigma factor protein and a cytoplasmic sigma factor protein. The structures of the intact TonB-dependent outer membrane receptor FecA from Escherichia coli and FpvA from Pseudomonas aeruginosa have recently been solved by protein crystallography; however, no electron density was detected for their periplasmic signalling domains, suggesting that it was either unfolded or flexible with respect to the remainder of the protein. Here we describe the well-defined solution structure of this domain solved by multidimensional nuclear magnetic resonance (NMR) spectroscopy. The monomeric protein construct contains the 79-residue N-terminal domain as well as the next 17 residues that are part of the receptor's plug domain. These form two clearly distinct regions: a highly structured domain at the N-terminal end followed by an extended flexible tail at the C-terminal end, which includes the 'TonB-box' region, and connects it to the plug domain of the receptor. The structured region consists of two alpha-helices that are positioned side by side and are sandwiched in between two small beta-sheets. This is a novel protein fold which appears to be preserved in all the periplasmic signalling domains of bacterial TonB-dependent outer membrane receptors that are involved in ECF signalling, because the hydrophobic residues that make up the core of the protein domain are highly conserved.
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d1/2d1u_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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2D1U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2D1U OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2d1u ConSurf].
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Nuclear magnetic resonance solution structure of the periplasmic signalling domain of the TonB-dependent outer membrane transporter FecA from Escherichia coli., Garcia-Herrero A, Vogel HJ, Mol Microbiol. 2005 Dec;58(5):1226-37. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16313612 16313612]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Garcia-Herrero, A.]]
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[[Category: Garcia-Herrero A]]
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[[Category: Vogel, H J.]]
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[[Category: Vogel HJ]]
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[[Category: Feca]]
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[[Category: Iron-uptake]]
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[[Category: Surface signaling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:32:08 2008''
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Current revision

Solution structure of the periplasmic signaling domain of FecA from Escherichia coli

PDB ID 2d1u

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