2ezr

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[[Image:2ezr.gif|left|200px]]<br /><applet load="2ezr" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2ezr" />
 
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'''SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 21-30 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES'''<br />
 
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==Overview==
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==SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 21-30 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES==
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The solution structure of the ectodomain of simian immunodeficiency virus (SIV) gp41 (e-gp41), consisting of residues 27-149, has been determined by multidimensional heteronuclear NMR spectroscopy. SIV e-gp41 is a symmetric 44 kDa trimer with each subunit consisting of antiparallel N-terminal (residues 30-80) and C-terminal (residues 107-147) helices connected by a 26 residue loop (residues 81-106). The N-terminal helices of each subunit form a parallel coiled-coil structure in the interior of the complex which is surrounded by the C-terminal helices located on the exterior of the complex. The loop region is ordered and displays numerous intermolecular and non-sequential intramolecular contacts. The helical core of SIV e-gp41 is similar to recent X-ray structures of truncated constructs of the helical core of HIV-1 e-gp41. The present structure establishes unambiguously the connectivity of the N- and C-terminal helices in the trimer, and characterizes the conformation of the intervening loop, which has been implicated by mutagenesis and antibody epitope mapping to play a key role in gp120 association. In conjunction with previous studies, the solution structure of the SIV e-gp41 ectodomain provides insight into the binding site of gp120 and the mechanism of cell fusion. The present structure of SIV e-gp41 represents one of the largest protein structures determined by NMR to date.
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<StructureSection load='2ezr' size='340' side='right'caption='[[2ezr]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2ezr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EZR FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ezr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ezr OCA], [https://pdbe.org/2ezr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ezr RCSB], [https://www.ebi.ac.uk/pdbsum/2ezr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ezr ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q88033_SIV Q88033_SIV]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ez/2ezr_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ezr ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2EZR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EZR OCA].
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*[[Gp41 3D Structures|Gp41 3D Structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Three-dimensional solution structure of the 44 kDa ectodomain of SIV gp41., Caffrey M, Cai M, Kaufman J, Stahl SJ, Wingfield PT, Covell DG, Gronenborn AM, Clore GM, EMBO J. 1998 Aug 17;17(16):4572-84. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9707417 9707417]
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[[Category: Large Structures]]
[[Category: Simian immunodeficiency virus]]
[[Category: Simian immunodeficiency virus]]
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[[Category: Single protein]]
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[[Category: Caffrey M]]
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[[Category: Caffrey, M.]]
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[[Category: Clore GM]]
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[[Category: Clore, G M.]]
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[[Category: Gronenborn AM]]
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[[Category: Gronenborn, A M.]]
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[[Category: siv gp41 ectodomain]]
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[[Category: virus envelope protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:16:13 2008''
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Current revision

SOLUTION NMR STRUCTURE OF ECTODOMAIN OF SIV GP41, MODELS 21-30 OF AN ENSEMBLE OF 40 SIMULATED ANNEALING STRUCTURES

PDB ID 2ezr

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