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2hsp

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[[Image:2hsp.gif|left|200px]]<br /><applet load="2hsp" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2hsp" />
 
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'''SOLUTION STRUCTURE OF THE SH3 DOMAIN OF PHOSPHOLIPASE CGAMMA'''<br />
 
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==Overview==
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==SOLUTION STRUCTURE OF THE SH3 DOMAIN OF PHOSPHOLIPASE CGAMMA==
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SH3 (Src homology 3) domains are found in many signaling proteins and appear to function as binding modules for cytoplasmic target proteins. The solution structure of the SH3 domain of human phospholipase C-gamma (PLC-gamma) was determined by two-dimensional 1H NMR analysis. This SH3 domain is composed of eight antiparallel beta strands consisting of two successive "Greek key" motifs, which form a barrel-like structure. The conserved aliphatic and aromatic residues form a hydrophobic pocket on the molecular surface, and the conserved carboxylic residues are localized to the periphery. The hydrophobic pocket may serve as a binding site for target proteins. Analysis of the slowly exchanging amide protons by NMR measurements indicates that despite containing a high content of beta structure, the SH3 domain of PLC-gamma is flexible.
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<StructureSection load='2hsp' size='340' side='right'caption='[[2hsp]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2hsp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hsp 1hsp]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2HSP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2hsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hsp OCA], [https://pdbe.org/2hsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2hsp RCSB], [https://www.ebi.ac.uk/pdbsum/2hsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2hsp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PLCG1_HUMAN PLCG1_HUMAN] Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand-mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration.<ref>PMID:17229814</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hs/2hsp_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2hsp ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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2HSP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1HSP. Active as [http://en.wikipedia.org/wiki/Phosphoinositide_phospholipase_C Phosphoinositide phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.11 3.1.4.11] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HSP OCA].
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*[[Phospholipase C|Phospholipase C]]
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== References ==
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==Reference==
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<references/>
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Solution structure of the SH3 domain of phospholipase C-gamma., Kohda D, Hatanaka H, Odaka M, Mandiyan V, Ullrich A, Schlessinger J, Inagaki F, Cell. 1993 Mar 26;72(6):953-60. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7681365 7681365]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Phosphoinositide phospholipase C]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Hatanaka H]]
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[[Category: Hatanaka, H.]]
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[[Category: Inagaki F]]
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[[Category: Inagaki, F.]]
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[[Category: Kohda D]]
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[[Category: Kohda, D.]]
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[[Category: Odaka M]]
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[[Category: Odaka, M.]]
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[[Category: phosphoric diester hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:45:17 2008''
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Current revision

SOLUTION STRUCTURE OF THE SH3 DOMAIN OF PHOSPHOLIPASE CGAMMA

PDB ID 2hsp

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