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2j5x

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[[Image:2j5x.gif|left|200px]]<br />
 
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<applet load="2j5x" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2j5x, resolution 2.80&Aring;" />
 
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'''STRUCTURE OF THE SMALL G PROTEIN ARF6 IN COMPLEX WITH GTPGAMMAS'''<br />
 
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==Overview==
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==STRUCTURE OF THE SMALL G PROTEIN ARF6 IN COMPLEX WITH GTPGAMMAS==
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The small GTP-binding protein Arf6 coordinates membrane traffic at the, plasma membrane with aspects of cytoskeleton organization. This function, does not overlap with that of other members of the ADP-ribosylation factor, (Arf) family, although their switch regions, which are their major sites, of interaction with regulators and effectors, have virtually identical, sequences. Here we report the crystal structure of full-length, non-myristoylated human Arf6 bound to GTPgammaS. Unlike their GDP-bound, forms, the active forms of Arf6 and Arf1 are very similar. Thus, the, switch regions are discriminatory elements between Arf isoforms in their, inactive but not in their active forms, a property that may generalize to, other families of small G proteins. This suggests that GTP-bound Arfs may, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11266366 (full description)]]
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<StructureSection load='2j5x' size='340' side='right'caption='[[2j5x]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2j5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hfv 1hfv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2J5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2J5X FirstGlance]. <br>
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2J5X is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with MG and GSP as [[http://en.wikipedia.org/wiki/ligands ligands]]. This structure superseeds the now removed PDB entry 1HFV. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2J5X OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2j5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j5x OCA], [https://pdbe.org/2j5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2j5x RCSB], [https://www.ebi.ac.uk/pdbsum/2j5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2j5x ProSAT]</span></td></tr>
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The structural GDP/GTP cycle of human Arf6., Pasqualato S, Menetrey J, Franco M, Cherfils J, EMBO Rep. 2001 Mar;2(3):234-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11266366 11266366]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ARF6_HUMAN ARF6_HUMAN] GTP-binding protein involved in protein trafficking; regulates endocytic recycling and cytoskeleton remodeling. May modulate vesicle budding and uncoating within the Golgi apparatus. Functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in the regulation of dendritic spine development (By similarity). Contributes to the regulation of dendritic branching and filopodia extension.<ref>PMID:7589240</ref> <ref>PMID:14978216</ref> <ref>PMID:11266366</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/j5/2j5x_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2j5x ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cherfils, J.]]
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[[Category: Cherfils J]]
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[[Category: Franco, M.]]
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[[Category: Franco M]]
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[[Category: Menetrey, J.]]
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[[Category: Menetrey J]]
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[[Category: Pasqualato, S.]]
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[[Category: Pasqualato S]]
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[[Category: GSP]]
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[[Category: MG]]
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[[Category: arf]]
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[[Category: arf6]]
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[[Category: er-golgi transport]]
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[[Category: g protein]]
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[[Category: golgi apparatus]]
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[[Category: gtp-binding]]
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[[Category: lipoprotein]]
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[[Category: membrane traffic]]
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[[Category: myristate]]
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[[Category: nucleotide-binding]]
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[[Category: protein transport]]
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[[Category: ras]]
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[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 11:33:16 2007''
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Current revision

STRUCTURE OF THE SMALL G PROTEIN ARF6 IN COMPLEX WITH GTPGAMMAS

PDB ID 2j5x

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