2jiy

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[[Image:2jiy.gif|left|200px]]<br />
 
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<applet load="2jiy" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2jiy, resolution 2.20&Aring;" />
 
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'''PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M149 REPLACED WITH TRP (CHAIN M, AM149W)'''<br />
 
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==Overview==
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==PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M149 REPLACED WITH TRP (CHAIN M, AM149W)==
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X-ray crystallography has been used to investigate the extent of, structural changes in mutants of the purple bacterial reaction center that, assemble without a particular ubiquinone or bacteriopheophytin cofactor., In the case of the bacteriopheophytin-exclusion mutant, in which Ala M149, was replaced by Trp (AM149W), the quality of protein crystals was improved, over that seen in previous work by minimizing illumination, time, and, temperature during the purification protocol and carrying out crystal, growth at 4 degrees C after overnight incubation at 18 degrees C. The, X-ray crystal structure of the AM149W mutant, determined to a resolution, of 2.2 A, showed very little change in protein structure despite the, absence of the bacteriopheophytin cofactor. Changes in the electron, ... [[http://ispc.weizmann.ac.il/pmbin/getpm?17711306 (full description)]]
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<StructureSection load='2jiy' size='340' side='right'caption='[[2jiy]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2jiy]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides Cereibacter sphaeroides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JIY FirstGlance]. <br>
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2JIY is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Rhodobacter_sphaeroides Rhodobacter sphaeroides]] with FE, PO4, CL, LDA, BCL, BPH, U10, CDL, SPN and D12 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2JIY OCA]].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jiy OCA], [https://pdbe.org/2jiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jiy RCSB], [https://www.ebi.ac.uk/pdbsum/2jiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jiy ProSAT]</span></td></tr>
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Structural Responses to Cavity-Creating Mutations in an Integral Membrane Protein(,)., Fyfe PK, Potter JA, Cheng J, Williams CM, Watson AJ, Jones MR, Biochemistry. 2007 Aug 21;. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17711306 17711306]
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</table>
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[[Category: Protein complex]]
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== Function ==
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[[Category: Rhodobacter sphaeroides]]
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[https://www.uniprot.org/uniprot/RCEH_CERSP RCEH_CERSP] The reaction center is a membrane-bound complex that mediates the initial photochemical event in the electron transfer process of photosynthesis.
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[[Category: Cheng, J.]]
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== Evolutionary Conservation ==
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[[Category: Fyfe, P.K.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Jones, M.R.]]
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Check<jmol>
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[[Category: Potter, J.]]
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<jmolCheckbox>
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[[Category: Watson, A.J.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ji/2jiy_consurf.spt"</scriptWhenChecked>
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[[Category: Williams, C.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: BCL]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: BPH]]
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</jmolCheckbox>
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[[Category: CDL]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2jiy ConSurf].
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[[Category: CL]]
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<div style="clear:both"></div>
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[[Category: D12]]
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__TOC__
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[[Category: FE]]
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</StructureSection>
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[[Category: LDA]]
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[[Category: Cereibacter sphaeroides]]
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[[Category: PO4]]
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[[Category: Large Structures]]
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[[Category: SPN]]
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[[Category: Cheng J]]
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[[Category: U10]]
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[[Category: Fyfe PK]]
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[[Category: bacteriochlorophyll]]
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[[Category: Jones MR]]
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[[Category: chlorophyll]]
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[[Category: Potter JA]]
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[[Category: chromophore]]
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[[Category: Watson AJ]]
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[[Category: electron transport]]
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[[Category: Williams CM]]
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[[Category: iron]]
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[[Category: magnesium]]
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[[Category: membrane]]
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[[Category: membrane protein]]
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[[Category: metal-binding]]
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[[Category: photosynthesis]]
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[[Category: photosynthetic reaction center]]
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[[Category: reaction center]]
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[[Category: transmembrane]]
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[[Category: transport]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:37:11 2007''
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Current revision

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M149 REPLACED WITH TRP (CHAIN M, AM149W)

PDB ID 2jiy

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