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2kv5

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==Solution structure of the par toxin Fst in DPC micelles==
==Solution structure of the par toxin Fst in DPC micelles==
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<StructureSection load='2kv5' size='340' side='right' caption='[[2kv5]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2kv5' size='340' side='right'caption='[[2kv5]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2kv5]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KV5 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2kv5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KV5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KV5 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv5 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kv5 RCSB], [http://www.ebi.ac.uk/pdbsum/2kv5 PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kv5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kv5 OCA], [https://pdbe.org/2kv5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kv5 RCSB], [https://www.ebi.ac.uk/pdbsum/2kv5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kv5 ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9RLG7_ENTFL Q9RLG7_ENTFL]
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The par toxin-antitoxin system is required for the stable inheritance of the plasmid pAD1 in its native host Enterococcus faecalis. It codes for the toxin Fst and a small antisense RNA which inhibits translation of toxin mRNA, and it is the only known antisense regulated toxin-antitoxin system in Gram-positive bacteria. This study presents the structure of the par toxin Fst, the first atomic resolution structure of a component of an antisense regulated toxin-antitoxin system. The mode of membrane binding was determined by relaxation enhancements in a paramagnetic environment and molecular dynamics simulation. Fst forms a membrane-binding alpha-helix in the N-terminal part and contains an intrinsically disordered region near the C-terminus. It binds in a transmembrane orientation with the C-terminus likely pointing toward the cytosol. Membrane-bound, alpha-helical peptides are frequently found in higher organisms as components of the innate immune system. Despite similarities to these antimicrobial peptides, Fst shows neither hemolytic nor antimicrobial activity when applied externally to a series of bacteria, fungal cells, and erythrocytes. Moreover, its charge distribution, orientation in the membrane, and structure distinguish it from antimicrobial peptides.
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Solution structure and membrane binding of the toxin fst of the par addiction module.,Gobl C, Kosol S, Stockner T, Ruckert HM, Zangger K Biochemistry. 2010 Aug 10;49(31):6567-75. PMID:20677831<ref>PMID:20677831</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Gobl, C]]
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[[Category: Enterococcus faecalis]]
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[[Category: Kosol, S]]
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[[Category: Large Structures]]
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[[Category: Ruckert, H M]]
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[[Category: Gobl C]]
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[[Category: Zangger, K]]
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[[Category: Kosol S]]
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[[Category: Bacterial]]
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[[Category: Ruckert HM]]
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[[Category: Toxin]]
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[[Category: Zangger K]]
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[[Category: Toxin-antitoxin]]
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Current revision

Solution structure of the par toxin Fst in DPC micelles

PDB ID 2kv5

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