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2kw0

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==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
==Solution structure of N-terminal domain of CcmH from Escherichia.coli==
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<StructureSection load='2kw0' size='340' side='right' caption='[[2kw0]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2kw0' size='340' side='right'caption='[[2kw0]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_bl21 Escherichia coli bl21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2kw0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21 Escherichia coli BL21]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KW0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KW0 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">b2194, B21_02080, ccmH, JW2182, yejP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=511693 Escherichia coli BL21])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [http://www.ebi.ac.uk/pdbsum/2kw0 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kw0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kw0 OCA], [https://pdbe.org/2kw0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kw0 RCSB], [https://www.ebi.ac.uk/pdbsum/2kw0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kw0 ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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In the Gram-negative bacterium of Escherichia coli, eight genes organized as a ccm operon (ccmABCDEFGH) are involved in the maturation of c-type cytochromes. The proteins encoded by the last three genes ccmFGH are believed to form a lyase complex functioning in the reduction of apocytochrome c and haem attachment. Among them, CcmH is a membrane-associated protein; its N-terminus is a catalytic domain with the active CXXC motif and the C-terminus is predicted as a TPR-like domain with unknown function. By using SCAM (scanning cysteine accessibility mutagenesis) and Gaussia luciferase fusion assays, we provide experimental evidence for the entire topological structure of E. coli CcmH. The mature CcmH is a periplasm-resident oxidoreductase anchored to the inner membrane by two transmembrane segments. Both N- and C-terminal domains are located and function in the periplasmic compartment. Moreover, the N-terminal domain forms a monomer in solution, while the C-terminal domain is a compact fold with helical structures. The NMR solution structure of the catalytic domain in reduced form exhibits mainly a three-helix bundle, providing further information for the redox mechanism. The redox potential suggests that CcmH exhibits a strong reductase that may function in the last step of reduction of apocytochrome c for haem attachment.
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Biochemical properties and catalytic domain structure of the CcmH protein from Escherichia coli.,Zheng XM, Hong J, Li HY, Lin DH, Hu HY Biochim Biophys Acta. 2012 Dec;1824(12):1394-400. doi:, 10.1016/j.bbapap.2012.06.017. Epub 2012 Jul 10. PMID:22789558<ref>PMID:22789558</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia coli bl21]]
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[[Category: Escherichia coli BL21]]
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[[Category: Hong, J.]]
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[[Category: Large Structures]]
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[[Category: Hu, H.]]
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[[Category: Hong J]]
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[[Category: Lin, D.]]
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[[Category: Hu H]]
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[[Category: Zheng, X.]]
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[[Category: Lin D]]
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[[Category: Cytochrome c maturation]]
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[[Category: Zheng X]]
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[[Category: Oxidoreductase]]
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Current revision

Solution structure of N-terminal domain of CcmH from Escherichia.coli

PDB ID 2kw0

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