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2kxr

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==ZO1 ZU5 domain MC/AA mutation==
==ZO1 ZU5 domain MC/AA mutation==
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<StructureSection load='2kxr' size='340' side='right' caption='[[2kxr]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2kxr' size='340' side='right'caption='[[2kxr]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2kxr]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KXR FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2kxr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KXR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KXR FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kxs|2kxs]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TJP1, ZO1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxr OCA], [https://pdbe.org/2kxr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kxr RCSB], [https://www.ebi.ac.uk/pdbsum/2kxr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kxr ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kxr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kxr OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2kxr RCSB], [http://www.ebi.ac.uk/pdbsum/2kxr PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/ZO1_HUMAN ZO1_HUMAN] The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells.<ref>PMID:21240187</ref>
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Zonula occludens (ZO)-1 is a multi-domain scaffold protein known to have critical roles in the establishment of cell-cell adhesions and the maintenance of stable tissue structures through the targeting, anchoring, and clustering of transmembrane adhesion molecules and cytoskeletal proteins. Here, we report that ZO-1 directly binds to MRCKbeta, a Cdc42 effector kinase that modulates cell protrusion and migration, at the leading edge of migrating cells. Structural studies reveal that the binding of a beta hairpin from GRINL1A converts ZO-1 ZU5 into a complete ZU5-fold. A similar interaction mode is likely to occur between ZO-1 ZU5 and MRCKbeta. The interaction between ZO-1 and MRCKbeta requires the kinase to be primed by Cdc42 due to the closed conformation of the kinase. Formation of the ZO-1/MRCKbeta complex enriches the kinase at the lamellae of migrating cells. Disruption of the ZO-1/MRCKbeta complex inhibits MRCKbeta-mediated cell migration. These results demonstrate that ZO-1, a classical scaffold protein with accepted roles in maintaining cell-cell adhesions in stable tissues, also has an active role in cell migration during processes such as tissue development and remodelling.
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Cdc42-dependent formation of the ZO-1/MRCKbeta complex at the leading edge controls cell migration.,Huo L, Wen W, Wang R, Kam C, Xia J, Feng W, Zhang M EMBO J. 2011 Feb 16;30(4):665-78. Epub 2011 Jan 14. PMID:21240187<ref>PMID:21240187</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Wen, W]]
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[[Category: Large Structures]]
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[[Category: Zhang, M]]
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[[Category: Wen W]]
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[[Category: Beta-barrel]]
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[[Category: Zhang M]]
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[[Category: Protein binding]]
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ZO1 ZU5 domain MC/AA mutation

PDB ID 2kxr

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