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2l4j

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==Yap ww2==
==Yap ww2==
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<StructureSection load='2l4j' size='340' side='right' caption='[[2l4j]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''>
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<StructureSection load='2l4j' size='340' side='right'caption='[[2l4j]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2l4j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Japanese_medaka Japanese medaka]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L4J FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2l4j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryzias_latipes Oryzias latipes]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L4J FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4j OCA], [http://pdbe.org/2l4j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l4j RCSB], [http://www.ebi.ac.uk/pdbsum/2l4j PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4j OCA], [https://pdbe.org/2l4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l4j RCSB], [https://www.ebi.ac.uk/pdbsum/2l4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4j ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/E7FH70_ORYLA E7FH70_ORYLA]
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The paralogous multifunctional adaptor proteins YAP and TAZ are the nuclear effectors of the Hippo pathway, a central mechanism of organ size control and stem cell self-renewal. WW domains, mediators of protein-protein interactions, are essential for YAP and TAZ function, enabling interactions with PPxY motifs of numerous partner proteins. YAP has single and double WW domain isoforms (YAP1 and YAP2) whereas only a single WW domain isoform of TAZ has been described to date. Here we identify the first example of a double WW domain isoform of TAZ. Using NMR, we have characterized conformational features and peptide binding of YAP and TAZ tandem WW domains (WW1-WW2). The solution structure of YAP WW2 confirms that it has a canonical three-stranded antiparallel beta-sheet WW domain fold. While chemical shift-based analysis indicates that the WW domains in the tandem WW pairs retain the characteristic WW domain fold, 15N relaxation data show that, within the respective WW pairs, YAP WW1 and both WW1 and WW2 of TAZ undergo conformational exchange. 15N relaxation data also indicate that the linker between the WW domains is flexible in both YAP and TAZ. Within both YAP and TAZ tandem WW pairs, WW1 and WW2 bind single PPxY-containing peptide ligand concurrently and noncooperatively with sub-mM affinity. YAP and TAZ WW1-WW2 bind a dual PPxY-containing peptide with approximately 6-fold higher affinity. Our results indicate that both WW domains in YAP and TAZ are functional and capable of enhanced affinity binding to multi-PPxY partner proteins such as LATS1, ErbB4, and AMOT.
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Structural features and ligand binding properties of tandem WW domains from YAP and TAZ, nuclear effectors of the Hippo pathway.,Webb C, Upadhyay A, Giuntini F, Eggleston I, Furutani-Seiki M, Ishima R, Bagby S Biochemistry. 2011 Apr 26;50(16):3300-9. Epub 2011 Mar 31. PMID:21417403<ref>PMID:21417403</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2l4j" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Japanese medaka]]
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[[Category: Large Structures]]
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[[Category: Bagby, S]]
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[[Category: Oryzias latipes]]
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[[Category: Furutani-Seiki, M]]
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[[Category: Bagby S]]
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[[Category: Upadhyay, A]]
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[[Category: Furutani-Seiki M]]
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[[Category: Webb, C]]
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[[Category: Upadhyay A]]
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[[Category: Medaka]]
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[[Category: Webb C]]
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[[Category: Transcription]]
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[[Category: Ww domain]]
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[[Category: Yap]]
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Current revision

Yap ww2

PDB ID 2l4j

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