2lnh

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==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
==Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly==
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<StructureSection load='2lnh' size='340' side='right'caption='[[2lnh]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2lnh' size='340' side='right'caption='[[2lnh]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Eco57 Eco57] and [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2lnh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LNH FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">WASL ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), BAIAP2L1, IRTKS ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), espF(U), tccP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83334 ECO57])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lnh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lnh OCA], [https://pdbe.org/2lnh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lnh RCSB], [https://www.ebi.ac.uk/pdbsum/2lnh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lnh ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN]] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref> [[https://www.uniprot.org/uniprot/ESFU3_ECO57 ESFU3_ECO57]] Required for efficient pedestal formation in host epithelial cells during infection. Acts as an intermediate between Tir (via host BAIAP2) and host WASL/N-WASP. Directly binds and activates WASL/N-WASP, which stimulates actin polymerization and leads to the formation of actin pedestals at the sites of bacterial adhesion.<ref>PMID:18650809</ref> <ref>PMID:22921828</ref> [[https://www.uniprot.org/uniprot/BI2L1_HUMAN BI2L1_HUMAN]] May function as adapter protein. Involved in the formation of clusters of actin bundles. Plays a role in the reorganization of the actin cytoskeleton in response to bacterial infection.<ref>PMID:17430976</ref> <ref>PMID:19366662</ref> <ref>PMID:22921828</ref>
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[https://www.uniprot.org/uniprot/WASL_HUMAN WASL_HUMAN] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.<ref>PMID:19366662</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Intrinsically disordered protein (IDP)-mediated interactions are often characterized by low affinity but high specificity. These traits are essential in signaling and regulation that require reversibility. Enterohaemorrhagic Escherichia coli (EHEC) exploit this situation by commandeering host cytoskeletal signaling to stimulate actin assembly beneath bound bacteria, generating "pedestals" that promote intestinal colonization. EHEC translocates two proteins, EspF(U) and Tir, which form a complex with the host protein IRTKS. The interaction of this complex with N-WASP triggers localized actin polymerization. We show that EspF(U) is an IDP that contains a transiently alpha-helical N-terminus and dynamic C-terminus. Our structure shows that single EspF(U) repeat forms a high-affinity trimolecular complex with N-WASP and IRTKS. We demonstrate that bacterial and cellular ligands interact with IRTKS SH3 in a similar fashion, but the bacterial protein has evolved to outcompete cellular targets by utilizing a tryptophan switch that offers superior binding affinity enabling EHEC-induced pedestal formation.
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Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly.,Aitio O, Hellman M, Skehan B, Kesti T, Leong JM, Saksela K, Permi P Structure. 2012 Aug 21. PMID:22921828<ref>PMID:22921828</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2lnh" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Wiskott-Aldrich syndrome protein|Wiskott-Aldrich syndrome protein]]
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*[[Wiskott-Aldrich syndrome protein 3D structures|Wiskott-Aldrich syndrome protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Eco57]]
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[[Category: Escherichia coli O157:H7]]
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[[Category: Human]]
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Aitio, O]]
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[[Category: Aitio O]]
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[[Category: Hellman, M]]
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[[Category: Hellman M]]
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[[Category: Kesti, T]]
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[[Category: Kesti T]]
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[[Category: Leong, J M]]
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[[Category: Leong JM]]
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[[Category: Permi, P]]
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[[Category: Permi P]]
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[[Category: Saksela, K]]
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[[Category: Saksela K]]
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[[Category: Skehan, B]]
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[[Category: Skehan B]]
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[[Category: Protein complex]]
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[[Category: Signaling protein-protein binding complex]]
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Current revision

Enterohaemorrhagic E. coli (EHEC) exploits a tryptophan switch to hijack host F-actin assembly

PDB ID 2lnh

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