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2lus
From Proteopedia
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== Function == | == Function == | ||
[https://www.uniprot.org/uniprot/A3E0V9_CARRO A3E0V9_CARRO] | [https://www.uniprot.org/uniprot/A3E0V9_CARRO A3E0V9_CARRO] | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Thioredoxins (Trxs), which play a key role in maintaining a redox environment in the cell, are found in almost all organisms. Trxs act as potential reducing agents of disulfide bonds and contain two vicinal cysteines in a CXXC motif at the active site. Trx is also known to activate the DNA binding activity of NF-kappaB, an important transcription factor. Previously, Trx-related protein 16 from Carcinoscorpius rotundicauda (Cr-TRP16), a 16-kDa Trx-like protein that contains a WCPPC motif, was reported. Here we present the NMR structure of the reduced form of Cr-TRP16, along with its regulation of NF-kappaB activity. Unlike other 16-kDa Trx-like proteins, Cr-TRP16 contains an additional Cys residue (Cys-15, at the N terminus), through which it forms a homodimer. Moreover, we have explored the molecular basis of Cr-TRP16-mediated activation of NF-kappaB and showed that Cr-TRP16 exists as a dimer under physiological conditions, and only the dimeric form binds to NF-kappaB and enhances its DNA binding activity by directly reducing the cysteines in the DNA-binding motif of NF-kappaB. The C15S mutant of Cr-TRP16 was unable to dimerize and hence does not bind to NF-kappaB. Based on our finding and combined with the literature, we propose a model of how Cr-TRP16 is likely to bind to NF-kappaB. These findings elucidate the molecular mechanism by which NF-kappaB activation is regulated through Cr-TRP16. | ||
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| - | NMR structure of Carcinoscorpius rotundicauda thioredoxin-related protein 16 and its role in regulating transcription factor NF-kappaB activity.,Giri PK, Jing-Song F, Shanmugam MK, Ding JL, Sethi G, Swaminathan K, Sivaraman J J Biol Chem. 2012 Aug 24;287(35):29417-28. doi: 10.1074/jbc.M112.379859. Epub, 2012 Jul 3. PMID:22763700<ref>PMID:22763700</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2lus" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
NMR structure of Carcinoscorpius rotundicauda thioredoxin related protein 16 and its role in regulating transcription factor NF-kB activity
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