This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2lwm
From Proteopedia
(Difference between revisions)
| Line 8: | Line 8: | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lwm OCA], [https://pdbe.org/2lwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lwm RCSB], [https://www.ebi.ac.uk/pdbsum/2lwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lwm ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lwm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lwm OCA], [https://pdbe.org/2lwm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lwm RCSB], [https://www.ebi.ac.uk/pdbsum/2lwm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lwm ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | The addition of hydroxyl radicals to the C8 position of guanine can lead to the formation of a 2,6-diamino-4-hydroxy-5-formamido-2'-deoxypyrimidine (Fapy-dG) lesion, whose endogenous levels in cellular DNA rival those of 8-oxo-7,8-dihydroxy-2'-deoxyguanosine. Despite its prevalence, the structure of duplex DNA containing Fapy-dG is unknown. We have prepared an undecameric duplex containing a centrally located beta-cFapy-dG residue paired to dC and determined its solution structure by high-resolution NMR spectroscopy and restrained molecular dynamic simulations. The damaged duplex adopts a right-handed helical structure with all residues in an anti conformation, forming Watson-Crick base pair alignments, and 2-deoxyribose conformations in the C2'-endo/C1'-exo range. The formamido group of Fapy rotates out of the pyrimidine plane and is present in the Z and E configurations that equilibrate with an approximate 2:1 population ratio. The two isomeric duplexes show similar lesion-induced deviations from a canonical B-from DNA conformation that are minor and limited to the central three-base-pair segment of the duplex, affecting the stacking interactions with the 5-lesion-neighboring residue. We discuss the implications of our observations for translesion synthesis during DNA replication and the recognition of Fapy-dG by DNA glycosylases. | ||
| - | |||
| - | Solution structure of duplex DNA containing a beta-Carba-Fapy-dG lesion.,Lukin M, Zaliznyak T, Attaluri S, Johnson F, de Los Santos C Chem Res Toxicol. 2012 Nov 19;25(11):2423-31. doi: 10.1021/tx300290b. Epub 2012, Aug 29. PMID:22897814<ref>PMID:22897814</ref> | ||
| - | |||
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 2lwm" style="background-color:#fffaf0;"></div> | ||
| - | == References == | ||
| - | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Solution Structure of Duplex DNA Containing a b-Carba-Fapy-dG Lesion
| |||||||||||
