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2m5a

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Protein A binding by an engineered Affibody molecule

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Retrieved from "http://52.214.119.220/wiki/index.php/2m5a"

Categories: Large Structures | Staphylococcus aureus | Synthetic construct | Hard T

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 ==Protein A binding by an engineered Affibody molecule==
-<StructureSection load='2m5a' size='340' side='right'caption='[[2m5a]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2m5a' size='340' side='right'caption='[[2m5a]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2m5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885] and [https://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5A FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2m5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2M5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2M5A FirstGlance]. <br>
-</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1h0t|1h0t]]</div></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">spa ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2m5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m5a OCA], [https://pdbe.org/2m5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2m5a RCSB], [https://www.ebi.ac.uk/pdbsum/2m5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2m5a ProSAT]</span></td></tr>
 </table>
-<div style="background-color:#fffaf0;">
+== Function ==
-== Publication Abstract from PubMed ==
+[https://www.uniprot.org/uniprot/SPA_STAAU SPA_STAAU]
-Affibody molecules are engineered binding proteins, in which the three-helix bundle motif of the Z domain derived from protein A is used as a scaffold for sequence variation. We used phage display to select Affibody binders to staphylococcal protein A itself. The best binder, called ZpA963, binds with similar affinity and kinetics to the five homologous E, D, A, B and C domains of protein A, and to a five-domain protein A construct with an average dissociation constant, KD, of approximately 20 nM. The structure of ZpA963 in complex with the Z domain shows that it interacts with a surface on Z that is identical in the five protein A domains, which explains the multi-domain affinity. This property allows for high-affinity binding by dimeric Affibody molecules that simultaneously engage two protein A domains in a complex. We studied two ZpA963 dimers in which the subunits were linked by a C-terminal disulfide in a symmetric dimer or head-to-tail in a fusion protein, respectively. The dimers both bind protein A with high affinity, very slow off-rates and with saturation-dependent kinetics that can be understood in terms of dimer binding to multiple sites. The head-to-tail (ZpA963)2htt dimer binds with an off-rate of koff &lt;/= 5 x 10-6 s-1 and an estimated KD &lt;/= 16 pM. The results illustrate how dimers of selected monomer binding proteins can provide an efficient route for engineering of high-affinity binders to targets that contain multiple homologous domains or repeated structural units.
-High-affinity binding to staphylococcal protein A by an engineered dimeric Affibody molecule.,Lindborg M, Dubnovitsky A, Olesen K, Bjorkman T, Abrahmsen L, Feldwisch J, Hard T Protein Eng Des Sel. 2013 Aug 7. PMID:23924760<ref>PMID:23924760</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 2m5a" style="background-color:#fffaf0;"></div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Synthetic construct sequences]]
+[[Category: Staphylococcus aureus]]
-[[Category: Hard, T]]
+[[Category: Synthetic construct]]
-[[Category: Affibody molecule]]
+[[Category: Hard T]]
-[[Category: Binding protein]]
-[[Category: Protein some]]
-[[Category: Protein binding]]
-[[Category: Protein engineering]]
-[[Category: Z domain]]

2m5a

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Protein A binding by an engineered Affibody molecule

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