1rtc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:1rtc.jpg|left|200px]]
[[Image:1rtc.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 1rtc |SIZE=350|CAPTION= <scene name='initialview01'>1rtc</scene>, resolution 2.3&Aring;
+
The line below this paragraph, containing "STRUCTURE_1rtc", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND=
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span>
+
or leave the SCENE parameter empty for the default display.
-
|GENE=
+
-->
-
|DOMAIN=
+
{{STRUCTURE_1rtc| PDB=1rtc | SCENE= }}
-
|RELATEDENTRY=
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rtc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rtc OCA], [http://www.ebi.ac.uk/pdbsum/1rtc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rtc RCSB]</span>
+
-
}}
+
'''THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS'''
'''THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS'''
Line 25: Line 22:
[[Category: Ricinus communis]]
[[Category: Ricinus communis]]
[[Category: Single protein]]
[[Category: Single protein]]
-
[[Category: rRNA N-glycosylase]]
+
[[Category: RRNA N-glycosylase]]
[[Category: Ernst, S.]]
[[Category: Ernst, S.]]
[[Category: Katzin, B J.]]
[[Category: Katzin, B J.]]
Line 31: Line 28:
[[Category: Monzingo, A F.]]
[[Category: Monzingo, A F.]]
[[Category: Robertus, J D.]]
[[Category: Robertus, J D.]]
-
[[Category: glycosidase]]
+
[[Category: Glycosidase]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 07:53:00 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:32:37 2008''
+

Revision as of 04:53, 3 May 2008

Image:1rtc.jpg

Template:STRUCTURE 1rtc

THE STRUCTURE OF RECOMBINANT RICIN A CHAIN AT 2.3 ANGSTROMS


Overview

The plant cytotoxin ricin is a heterodimer with a cell surface binding (B) chain and an enzymatically active A chain (RTA) known to act as a specific N-glycosidase. RTA must be separated from B chain to attack rRNA. The X-ray structure of ricin has been solved recently; here we report the structure of the isolated A chain expressed from a clone in Escherichia coli. This structure of wild-type rRTA has and will continue to serve as the parent compound for difference Fouriers used to assess the structure of site-directed mutants designed to analyze the mechanism of this medically and commercially important toxin. The structure of the recombinant protein, rRTA, is virtually identical to that seen previously for A chain in the heterodimeric toxin. Some minor conformational changes due to interactions with B chain and to crystal packing differences are described. Perhaps the most significant difference is the presence in rRTA of an additional active site water. This molecule is positioned to act as the ultimate nucleophile in the depurination reaction mechanism proposed by Monzingo and Robertus (1992, J. Mol. Biol. 227, 1136-1145).

About this Structure

1RTC is a Single protein structure of sequence from Ricinus communis. Full crystallographic information is available from OCA.

Reference

Structure of recombinant ricin A chain at 2.3 A., Mlsna D, Monzingo AF, Katzin BJ, Ernst S, Robertus JD, Protein Sci. 1993 Mar;2(3):429-35. PMID:8453380 Page seeded by OCA on Sat May 3 07:53:00 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rtc"
Personal tools