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2mex

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==Structure of the tetrameric building block of the Salmonella Typhimurium PrgI Type three secretion system needle==
==Structure of the tetrameric building block of the Salmonella Typhimurium PrgI Type three secretion system needle==
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<StructureSection load='2mex' size='340' side='right'caption='[[2mex]], [[NMR_Ensembles_of_Models | 15 NMR models]]' scene=''>
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<StructureSection load='2mex' size='340' side='right'caption='[[2mex]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2mex]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salty Salty]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2mex]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._LT2 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MEX FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2lpz|2lpz]]</div></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prgI, STM2873 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mex OCA], [https://pdbe.org/2mex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mex RCSB], [https://www.ebi.ac.uk/pdbsum/2mex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mex ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mex OCA], [https://pdbe.org/2mex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mex RCSB], [https://www.ebi.ac.uk/pdbsum/2mex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mex ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY]] Required for invasion of epithelial cells.
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[https://www.uniprot.org/uniprot/PRGI_SALTY PRGI_SALTY] Required for invasion of epithelial cells.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Noncovalent supramolecular assemblies possess in general several unique subunit-subunit interfaces.The basic building block of such an assembly consists of several subunits and contains all unique interfaces. Atomic-resolution structures of monomeric subunits are typically accessed by crystallography or solution NMR and fitted into electron microscopy density maps. However, the structure of the intact building block in the assembled state remains unknown with this hybrid approach. Here, we present the solid-state NMR atomic structure of the building block of the type III secretion system needle. The building block structure consists of a homotetrameric subunit complex with three unique supramolecular interfaces. Side-chain positions at the interfaces were solved at atomic detail. The high-resolution structure reveals unambiguously the helical handedness of the assembly, determined to be right-handed for the type III secretion system needle.Additionally, the axial rise per subunit could be extracted from the tetramer structure and independently validated by mass-per-length measurements.
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Atomic structure and handedness of the building block of a biological assembly.,Loquet A, Habenstein B, Chevelkov V, Vasa SK, Giller K, Becker S, Lange A J Am Chem Soc. 2013 Dec 26;135(51):19135-8. doi: 10.1021/ja411362q. Epub 2013 Dec, 17. PMID:24328247<ref>PMID:24328247</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2mex" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Salty]]
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[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2]]
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[[Category: Becker, S]]
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[[Category: Becker S]]
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[[Category: Chevelkov, V]]
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[[Category: Chevelkov V]]
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[[Category: Giller, K]]
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[[Category: Giller K]]
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[[Category: Habenstein, B]]
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[[Category: Habenstein B]]
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[[Category: Lange, A]]
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[[Category: Lange A]]
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[[Category: Loquet, A]]
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[[Category: Loquet A]]
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[[Category: Filament]]
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[[Category: Needle]]
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[[Category: Protein transport]]
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[[Category: Secretion]]
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Current revision

Structure of the tetrameric building block of the Salmonella Typhimurium PrgI Type three secretion system needle

PDB ID 2mex

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