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2mfc

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{{STRUCTURE_2mfc| PDB=2mfc | SCENE= }}
 
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===Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL1)/RsmE(dimer) 2:1 complex===
 
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==Function==
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==Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL1)/RsmE(dimer) 2:1 complex==
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[[http://www.uniprot.org/uniprot/Q5MXB2_PSEFL Q5MXB2_PSEFL]] Could accelerate the degradation of some genes transcripts potentially through selective RNA binding (By similarity).[HAMAP-Rule:MF_00167][SAAS:SAAS003751_004_007709]
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<StructureSection load='2mfc' size='340' side='right'caption='[[2mfc]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2mfc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2MFC FirstGlance]. <br>
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[[2mfc]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MFC OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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[[Category: Allain, F H.T.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2mfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mfc OCA], [https://pdbe.org/2mfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2mfc RCSB], [https://www.ebi.ac.uk/pdbsum/2mfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2mfc ProSAT]</span></td></tr>
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[[Category: Duss, O.]]
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</table>
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[[Category: Konte, N Diarra Dit.]]
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== Function ==
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[[Category: Michel, E.]]
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[https://www.uniprot.org/uniprot/CSRA1_PSEPH CSRA1_PSEPH] A translational regulator that binds mRNA to regulate translation initiation and/or mRNA stability (PubMed:17704818, PubMed:23635605). Post-transcriptionally represses the expression of genes controlled by GacA/GacS (PubMed:15601712, PubMed:23635605). Binds the 5' UTR of mRNA; the mRNA binds to the outside edge to each monomer and each dimer could bind the same mRNA twice (PubMed:17704818). Recognizes a (A/U)CANGGANG(U/A) consensus, binds to GGA (part of the Shine-Dalgarno sequence) in the 5' UTR loop, which prevents ribosome binding (PubMed:17704818, PubMed:24561806, PubMed:23635605). Overexpression represses target protein expression; mutating nucleotides in the 5' UTR abolishes repression in vivo (PubMed:17704818, PubMed:23635605). Binds specifically to small RNAs (sRNA) RsmX, RsmZ and RsmY; these sRNAs fold into secondary structures with multiple GGA sequences in loops to which the CsrA proteins bind (PubMed:15601712, PubMed:16286659, PubMed:24828038). Binding to RsmX, RsmY or RsmZ titrates the protein so that it can no longer bind mRNA and repress translation (PubMed:15601712, PubMed:24828038). RsmZ can bind up to 5 CsrA1 (rsmE) dimers; they bind cooperatively to GGA sequences in RsmZ in a defined order (PubMed:24828038, PubMed:24561806). Required for optimal expression and stability of sRNAs RsmX, RsmY and RsmZ (PubMed:15601712, PubMed:16286659). Four CsrA1 dimers maximally protect RsmZ from RNase activity (PubMed:24828038). Deletion of rsmX, rsmY or rsmZ alone has no detectable phenotype, but a double rsmY-rsmZ deletion has a marked decrease in production of secondary metabolites HCN, exoprotease AprA, antifungal agent 2,4-diacetylphloroglucinol and swarming motility, and protects cucumber plants from fungal infection less well than wild-type; the triple sRNA deletion has even stronger loss of these phenotypes (PubMed:16286659).<ref>PMID:15601712</ref> <ref>PMID:16286659</ref> <ref>PMID:17704818</ref> <ref>PMID:23635605</ref> <ref>PMID:24561806</ref> <ref>PMID:24828038</ref>
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[[Category: Schubert, M.]]
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== References ==
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[[Category: Bacterial protein]]
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<references/>
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[[Category: Csra]]
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__TOC__
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[[Category: Csrb]]
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</StructureSection>
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[[Category: Messenger rna]]
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[[Category: Large Structures]]
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[[Category: Modulation of binding affinity]]
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[[Category: Pseudomonas fluorescens]]
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[[Category: Molecular mimicry]]
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[[Category: Allain FH-T]]
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[[Category: Non-coding rna]]
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[[Category: Diarra Dit Konte N]]
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[[Category: Protein sequestration]]
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[[Category: Duss O]]
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[[Category: Pseudomonas aeruginosa]]
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[[Category: Michel E]]
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[[Category: Rna-binding protein]]
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[[Category: Schubert M]]
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[[Category: Rsma]]
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[[Category: Rsme]]
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[[Category: Rsmz]]
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[[Category: Srna]]
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[[Category: Translation activation]]
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[[Category: Translation repressor protein]]
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[[Category: Translation-rna complex]]
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Current revision

Csr/Rsm protein-RNA recognition - A molecular affinity ruler: RsmZ(SL1)/RsmE(dimer) 2:1 complex

PDB ID 2mfc

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