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2nar

From Proteopedia

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Current revision

Solution structure of AVR3a_60-147 from Phytophthora infestans

Structural highlights

2nar is a 1 chain structure with sequence from Phytophthora infestans. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A3AKI_PHYIN Multifunctional effector that can suppress host BAK1/SERK3-mediated immunity through at least two different pathways (PubMed:19794118, PubMed:20457921, PubMed:21348873, PubMed:26348328). Manipulates plant immunity by targeting and stabilizing host E3 ligase CMPG1. Preventing the normal 26S proteasome-dependent degradation of potato CMPG1, and thus potentially of its protein substrates in the host cell, further abolishes host cell death during the biotrophic phase of infection (PubMed:19794118, PubMed:20457921, PubMed:21348873). Associates also with the dynamin-related protein 2 (DRP2), a plant GTPase involved in immune receptor-mediated endocytosis (PubMed:26348328). The Avr3A(KI) form is recognized by R3a which triggers R3a-mediated hypersensitivity and suppresses INF1-induced cell death (PubMed:15894622, PubMed:16965554, PubMed:19245321).^[1] ^[2] ^[3] ^[4] ^[5] ^[6] ^[7]

References

↑ Armstrong MR, Whisson SC, Pritchard L, Bos JI, Venter E, Avrova AO, Rehmany AP, Böhme U, Brooks K, Cherevach I, Hamlin N, White B, Fraser A, Lord A, Quail MA, Churcher C, Hall N, Berriman M, Huang S, Kamoun S, Beynon JL, Birch PR. An ancestral oomycete locus contains late blight avirulence gene Avr3a, encoding a protein that is recognized in the host cytoplasm. Proc Natl Acad Sci U S A. 2005 May 24;102(21):7766-71. PMID:15894622 doi:10.1073/pnas.0500113102
↑ Bos JI, Kanneganti TD, Young C, Cakir C, Huitema E, Win J, Armstrong MR, Birch PR, Kamoun S. The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana. Plant J. 2006 Oct;48(2):165-76. PMID:16965554 doi:10.1111/j.1365-313X.2006.02866.x
↑ Bos JI, Chaparro-Garcia A, Quesada-Ocampo LM, McSpadden Gardener BB, Kamoun S. Distinct amino acids of the Phytophthora infestans effector AVR3a condition activation of R3a hypersensitivity and suppression of cell death. Mol Plant Microbe Interact. 2009 Mar;22(3):269-81. PMID:19245321 doi:10.1094/MPMI-22-3-0269
↑ Oh SK, Young C, Lee M, Oliva R, Bozkurt TO, Cano LM, Win J, Bos JI, Liu HY, van Damme M, Morgan W, Choi D, Van der Vossen EA, Vleeshouwers VG, Kamoun S. In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2. Plant Cell. 2009 Sep;21(9):2928-47. PMID:19794118 doi:10.1105/tpc.109.068247
↑ Bos JI, Armstrong MR, Gilroy EM, Boevink PC, Hein I, Taylor RM, Zhendong T, Engelhardt S, Vetukuri RR, Harrower B, Dixelius C, Bryan G, Sadanandom A, Whisson SC, Kamoun S, Birch PR. Phytophthora infestans effector AVR3a is essential for virulence and manipulates plant immunity by stabilizing host E3 ligase CMPG1. Proc Natl Acad Sci U S A. 2010 May 25;107(21):9909-14. PMID:20457921 doi:10.1073/pnas.0914408107
↑ Gilroy EM, Taylor RM, Hein I, Boevink P, Sadanandom A, Birch PR. CMPG1-dependent cell death follows perception of diverse pathogen elicitors at the host plasma membrane and is suppressed by Phytophthora infestans RXLR effector AVR3a. New Phytol. 2011 May;190(3):653-66. PMID:21348873 doi:10.1111/j.1469-8137.2011.03643.x
↑ Chaparro-Garcia A, Schwizer S, Sklenar J, Yoshida K, Petre B, Bos JI, Schornack S, Jones AM, Bozkurt TO, Kamoun S. Phytophthora infestans RXLR-WY Effector AVR3a Associates with Dynamin-Related Protein 2 Required for Endocytosis of the Plant Pattern Recognition Receptor FLS2. PLoS One. 2015 Sep 8;10(9):e0137071. PMID:26348328 doi:10.1371/journal.pone.0137071

1 Structural highlights
2 Function
3 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2nar"

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 2nar is ON HOLD
+==Solution structure of AVR3a_60-147 from Phytophthora infestans==
+<StructureSection load='2nar' size='340' side='right'caption='[[2nar]]' scene=''>
-Authors: Matena, A., Bayer, P., Zhukov, I., Stanek, J., Kozminski, W., van West, P., Wawra, S.
+== Structural highlights ==
+<table><tr><td colspan='2'>[[2nar]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Phytophthora_infestans Phytophthora infestans]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2NAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2NAR FirstGlance]. <br>
-Description: Solution structure of AVR3a_60-147 from Phytophthora infestans
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-[[Category: Unreleased Structures]]
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2nar FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2nar OCA], [https://pdbe.org/2nar PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2nar RCSB], [https://www.ebi.ac.uk/pdbsum/2nar PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2nar ProSAT]</span></td></tr>
-[[Category: Zhukov, I]]
+</table>
-[[Category: Bayer, P]]
+== Function ==
-[[Category: Kozminski, W]]
+[https://www.uniprot.org/uniprot/A3AKI_PHYIN A3AKI_PHYIN] Multifunctional effector that can suppress host BAK1/SERK3-mediated immunity through at least two different pathways (PubMed:19794118, PubMed:20457921, PubMed:21348873, PubMed:26348328). Manipulates plant immunity by targeting and stabilizing host E3 ligase CMPG1. Preventing the normal 26S proteasome-dependent degradation of potato CMPG1, and thus potentially of its protein substrates in the host cell, further abolishes host cell death during the biotrophic phase of infection (PubMed:19794118, PubMed:20457921, PubMed:21348873). Associates also with the dynamin-related protein 2 (DRP2), a plant GTPase involved in immune receptor-mediated endocytosis (PubMed:26348328). The Avr3A(KI) form is recognized by R3a which triggers R3a-mediated hypersensitivity and suppresses INF1-induced cell death (PubMed:15894622, PubMed:16965554, PubMed:19245321).<ref>PMID:15894622</ref> <ref>PMID:16965554</ref> <ref>PMID:19245321</ref> <ref>PMID:19794118</ref> <ref>PMID:20457921</ref> <ref>PMID:21348873</ref> <ref>PMID:26348328</ref>
-[[Category: Matena, A]]
+== References ==
-[[Category: Wawra, S]]
+<references/>
-[[Category: Van West, P]]
+__TOC__
-[[Category: Stanek, J]]
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Phytophthora infestans]]
+[[Category: Bayer P]]
+[[Category: Kozminski W]]
+[[Category: Matena A]]
+[[Category: Stanek J]]
+[[Category: Wawra S]]
+[[Category: Zhukov I]]
+[[Category: Van West P]]

2nar

From Proteopedia

Current revision

Solution structure of AVR3a_60-147 from Phytophthora infestans

Structural highlights

Function

References

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Proteopedia Page Contributors and Editors (what is this?)

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