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2rt3

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==Solution structure of the second RRM domain of Nrd1==
==Solution structure of the second RRM domain of Nrd1==
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<StructureSection load='2rt3' size='340' side='right' caption='[[2rt3]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
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<StructureSection load='2rt3' size='340' side='right'caption='[[2rt3]]' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[2rt3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RT3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2RT3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[2rt3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RT3 FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">nrd1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rt3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2rt3 RCSB], [http://www.ebi.ac.uk/pdbsum/2rt3 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rt3 OCA], [https://pdbe.org/2rt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rt3 RCSB], [https://www.ebi.ac.uk/pdbsum/2rt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rt3 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/NRD1_SCHPO NRD1_SCHPO]] Negative regulator of sexual differentiation. Acts by repressing the transcription of meiosis-inducing, ste11-regulated genes until cells reach a critical level of starvation. RNA-binding protein that preferentially binds poly(U).<ref>PMID:15166138</ref>
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[https://www.uniprot.org/uniprot/NRD1_SCHPO NRD1_SCHPO] Negative regulator of sexual differentiation. Acts by repressing the transcription of meiosis-inducing, ste11-regulated genes until cells reach a critical level of starvation. RNA-binding protein that preferentially binds poly(U).<ref>PMID:15166138</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Negative regulator of differentiation 1 (Nrd1) is known as a negative regulator of sexual differentiation in fission yeast. Recently, it has been revealed that Nrd1 also regulates cytokinesis, in which physical separation of the cell is achieved by a contractile ring comprising many proteins including actin and myosin. Cdc4, a myosin II light chain, is known to be required for cytokinesis. Nrd1 binds and stabilizes Cdc4 mRNA, and thereby suppressing the cytokinesis defects of the cdc4 mutants. Interestingly, Pmk1 MAPK phosphorylates Nrd1, resulting in markedly reduced RNA binding activity. Furthermore, Nrd1 localizes to stress granules in response to various stresses, and Pmk1 phosphorylation enhances the localization. Nrd1 consists of four RRM domains, although the mechanism by which Pmk1 regulates the RNA binding activity of Nrd1 is unknown. In an effort to delineate the relationship between Nrd1 structure and function, we prepared each RNA binding domain of Nrd1 and examined RNA binding to chemically synthesized oligo RNA using NMR. The structure of the second RRM domain of Nrd1 was determined and the RNA binding site on the second RRM domain was mapped by NMR. A plausible mechanism pertaining to the regulation of RNA binding activity by phosphorylation is also discussed.
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Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation.,Kobayashi A, Kanaba T, Satoh R, Fujiwara T, Ito Y, Sugiura R, Mishima M Biochem Biophys Res Commun. 2013 Jul 19;437(1):12-7. doi:, 10.1016/j.bbrc.2013.06.008. Epub 2013 Jun 12. PMID:23770370<ref>PMID:23770370</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Fission yeast]]
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[[Category: Large Structures]]
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[[Category: Kanaba, T]]
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[[Category: Schizosaccharomyces pombe 972h-]]
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[[Category: Kobayashi, A]]
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[[Category: Kanaba T]]
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[[Category: Mishima, M]]
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[[Category: Kobayashi A]]
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[[Category: Nrd1]]
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[[Category: Mishima M]]
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[[Category: Rna binding protein]]
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[[Category: Rna-binding]]
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Current revision

Solution structure of the second RRM domain of Nrd1

PDB ID 2rt3

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