This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4afa

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:14, 1 May 2024) (edit) (undo)
 
(4 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal Structure of subtype-switched Epithelial Adhesin 1 to 2 A domain (Epa1to2A) from Candida glabrata in complex with glycerol==
==Crystal Structure of subtype-switched Epithelial Adhesin 1 to 2 A domain (Epa1to2A) from Candida glabrata in complex with glycerol==
-
<StructureSection load='4afa' size='340' side='right' caption='[[4afa]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
+
<StructureSection load='4afa' size='340' side='right'caption='[[4afa]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4afa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4AFA FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4afa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_glabrata Candida glabrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AFA FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05&#8491;</td></tr>
-
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4a3x|4a3x]], [[4af9|4af9]], [[4afb|4afb]], [[4afc|4afc]], [[4asl|4asl]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4afa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4afa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4afa RCSB], [http://www.ebi.ac.uk/pdbsum/4afa PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4afa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4afa OCA], [https://pdbe.org/4afa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4afa RCSB], [https://www.ebi.ac.uk/pdbsum/4afa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4afa ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q6VBJ0_CANGB Q6VBJ0_CANGB]
-
The human pathogenic yeast Candida glabrata harbors more than 20 surface-exposed, epithelial adhesins (Epas) for host cell adhesion. The Epa family recognizes host glycans and discriminates between target tissues by their adhesin (A) domains, but a detailed structural basis for ligand-binding specificity of Epa proteins has been lacking so far. In this study, we provide high-resolution crystal structures of the Epa1A domain in complex with different carbohydrate ligands that reveal how host cell mucin-type O-glycans are recognized and allow a structure-guided classification of the Epa family into specific subtypes. Further detailed structural and functional characterization of subtype-switched Epa1 variants shows that specificity is governed by two inner loops, CBL1 and CBL2, involved in calcium binding as well as by three outer loops, L1, L2, and L3. In summary, our study provides the structural basis for promiscuity and specificity of Epa adhesins, which might further contribute to developing anti-adhesive antimycotics and combating Candida colonization.
+
-
 
+
-
Structural basis for promiscuity and specificity during Candida glabrata invasion of host epithelia.,Maestre-Reyna M, Diderrich R, Veelders MS, Eulenburg G, Kalugin V, Bruckner S, Keller P, Rupp S, Mosch HU, Essen LO Proc Natl Acad Sci U S A. 2012 Oct 3. PMID:23035251<ref>PMID:23035251</ref>
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
==See Also==
-
</div>
+
*[[Adhesin 3D structures|Adhesin 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Candida glabrata]]
+
[[Category: Large Structures]]
-
[[Category: Brueckner, S]]
+
[[Category: Brueckner S]]
-
[[Category: Diderrich, R]]
+
[[Category: Diderrich R]]
-
[[Category: Essen, L O]]
+
[[Category: Essen L-O]]
-
[[Category: Eulenburg, G]]
+
[[Category: Eulenburg G]]
-
[[Category: Kalugin, V]]
+
[[Category: Kalugin V]]
-
[[Category: Keller, P]]
+
[[Category: Keller P]]
-
[[Category: Maestre-Reyna, M]]
+
[[Category: Maestre-Reyna M]]
-
[[Category: Moesch, H U]]
+
[[Category: Moesch H-U]]
-
[[Category: Rupp, S]]
+
[[Category: Rupp S]]
-
[[Category: Veelders, M S]]
+
[[Category: Veelders MS]]
-
[[Category: Cell adhesion]]
+
-
[[Category: Lectin]]
+
-
[[Category: Pathogenicity]]
+
-
[[Category: Tissue invasion]]
+

Current revision

Crystal Structure of subtype-switched Epithelial Adhesin 1 to 2 A domain (Epa1to2A) from Candida glabrata in complex with glycerol

PDB ID 4afa

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4afa"
Personal tools