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5nqf

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==Crystal structure of Plasmodium falciparum AMA1 in complex with a 39 aa PvRON2 peptide==
==Crystal structure of Plasmodium falciparum AMA1 in complex with a 39 aa PvRON2 peptide==
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<StructureSection load='5nqf' size='340' side='right' caption='[[5nqf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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<StructureSection load='5nqf' size='340' side='right'caption='[[5nqf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5nqf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5NQF FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5nqf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_Vietnam_Oak-Knoll_(FVO) Plasmodium falciparum Vietnam Oak-Knoll (FVO)] and [https://en.wikipedia.org/wiki/Plasmodium_vivax_Sal-1 Plasmodium vivax Sal-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NQF FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5nqg|5nqg]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5nqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nqf OCA], [http://pdbe.org/5nqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5nqf RCSB], [http://www.ebi.ac.uk/pdbsum/5nqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5nqf ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5nqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5nqf OCA], [https://pdbe.org/5nqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5nqf RCSB], [https://www.ebi.ac.uk/pdbsum/5nqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5nqf ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A0A024UZE1_PLAFA A0A024UZE1_PLAFA]
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Malaria, a disease endemic in many tropical and subtropical regions, is caused by infection of the erythrocyte by the apicomplexan parasite Plasmodium. Host-cell invasion is a complex process but two Plasmodium proteins, Apical Membrane Antigen 1 (AMA1) and the Rhoptry Neck protein complex (RON), play a key role. AMA1, present on the surface of the parasite, binds tightly to the RON2 component of the RON protein complex, which is inserted into the erythrocyte membrane during invasion. Blocking the AMA1-RON2 interaction with antibodies or peptides inhibits invasion, underlining its importance in the Plasmodium life cycle and as a target for therapeutic strategies. We describe the crystal structure of the complex formed between AMA1 from P. vivax (PvAMA1) and a peptide derived from the externally exposed region of P. vivax RON2 (PvRON2sp1), and of the heterocomplex formed between P. falciparum AMA1 (PfAMA1) and PvRON2sp1. Binding studies show that the affinity of PvRON2sp1 for PvAMA1 is weaker than that previously reported for the PfRON2sp1-PfAMA1 association. Moreover, while PvRON2sp1 shows strong cross-reactivity with PfAMA1, PfRON2sp1 displays no detectable interaction with PvAMA1. The structures show that the equivalent residues PvRON2-Thr2055 and PfRON2-Arg2041 largely account for this pattern of reactivity.
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Cross-reactivity between apical membrane antgen 1 and rhoptry neck protein 2 in P. vivax and P. falciparum: A structural and binding study.,Vulliez-Le Normand B, Saul FA, Hoos S, Faber BW, Bentley GA PLoS One. 2017 Aug 17;12(8):e0183198. doi: 10.1371/journal.pone.0183198., eCollection 2017. PMID:28817634<ref>PMID:28817634</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5nqf" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bentley, G A]]
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[[Category: Large Structures]]
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[[Category: Faber, B W]]
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[[Category: Plasmodium vivax Sal-1]]
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[[Category: Normand, B Vulliez-le]]
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[[Category: Bentley GA]]
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[[Category: Saul, F A]]
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[[Category: Faber BW]]
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[[Category: Ama1]]
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[[Category: Saul FA]]
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[[Category: Cell invasion]]
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[[Category: Vulliez-le Normand B]]
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[[Category: Malaria]]
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[[Category: Ron2]]
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Current revision

Crystal structure of Plasmodium falciparum AMA1 in complex with a 39 aa PvRON2 peptide

PDB ID 5nqf

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