This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


6fx3

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (07:33, 1 May 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==crystal structure of Pholiota squarrosa lectin in complex with a dodecasaccharide==
==crystal structure of Pholiota squarrosa lectin in complex with a dodecasaccharide==
-
<StructureSection load='6fx3' size='340' side='right' caption='[[6fx3]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+
<StructureSection load='6fx3' size='340' side='right'caption='[[6fx3]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[6fx3]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FX3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FX3 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[6fx3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pholiota_squarrosa Pholiota squarrosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FX3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6FX3 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=C4W:1-azido-beta-N-acetyl-D-glucosamine'>C4W</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fx3 OCA], [http://pdbe.org/6fx3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fx3 RCSB], [http://www.ebi.ac.uk/pdbsum/6fx3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fx3 ProSAT]</span></td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=C4W:1-azido-beta-N-acetyl-D-glucosamine'>C4W</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SIA:O-SIALIC+ACID'>SIA</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6fx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fx3 OCA], [https://pdbe.org/6fx3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6fx3 RCSB], [https://www.ebi.ac.uk/pdbsum/6fx3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6fx3 ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/A0A3B6UEU4_9AGAR A0A3B6UEU4_9AGAR]
-
We recombinantly produced and characterized the mini fungal lectin PhoSL. Despite a length of only 40 amino acids, PhoSL exclusively recognizes N-glycans with beta1,6-linked fucose. Core fucosylation influences the intrinsic properties and bioactivities of mammalian N-glycoproteins and its level is linked to various cancers. Thus, PhoSL serves as a promising tool for glycoprofiling. Without structural precedence, we solved its crystal structure using the zinc anomalous signal. We revealed an interlaced trimer creating a novel protein fold termed beta-prism III. Three biantennary core fucosylated N-glycan azides of 8 to 12 sugars were cocrystallized with PhoSL. The resulting highly resolved structures gave a detailed view on how the exclusive recognition of alpha1,6-fucosylated N-glycans by such a small protein occurs. This work also provided a protein consensus motif for the observed specificity as well as a glimpse on N-glycan flexibility upon binding.
+
-
 
+
-
Recognition of complex core fucosylated N-glycans by a mini lectin.,Cabanettes A, Perkams L, Spies C, Unverzagt C, Varrot A Angew Chem Int Ed Engl. 2018 Jun 29. doi: 10.1002/anie.201805165. PMID:29956878<ref>PMID:29956878</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 6fx3" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Cabanettes, A]]
+
[[Category: Large Structures]]
-
[[Category: Varrot, A]]
+
[[Category: Pholiota squarrosa]]
-
[[Category: Dodecasaccharide]]
+
[[Category: Cabanettes A]]
-
[[Category: Lectin]]
+
[[Category: Varrot A]]
-
[[Category: Sugar binding protein]]
+

Current revision

crystal structure of Pholiota squarrosa lectin in complex with a dodecasaccharide

PDB ID 6fx3

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6fx3"
Personal tools