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6hau

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<StructureSection load='6hau' size='340' side='right'caption='[[6hau]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
<StructureSection load='6hau' size='340' side='right'caption='[[6hau]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6hau]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Herpesvirus_saimiri Herpesvirus saimiri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HAU FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6hau]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Herpesvirus_saimiri_(strain_11) Herpesvirus saimiri (strain 11)] and [https://en.wikipedia.org/wiki/Human_gammaherpesvirus_8 Human gammaherpesvirus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HAU FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">EJRF1, ORF57 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10383 Herpesvirus saimiri])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hau OCA], [http://pdbe.org/6hau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hau RCSB], [http://www.ebi.ac.uk/pdbsum/6hau PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hau ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hau OCA], [https://pdbe.org/6hau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hau RCSB], [https://www.ebi.ac.uk/pdbsum/6hau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hau ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/ICP27_SHV21 ICP27_SHV21]] Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes (By similarity).
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[https://www.uniprot.org/uniprot/ICP27_SHV21 ICP27_SHV21] Early protein that promotes the accumulation and nuclear export of viral intronless RNA transcripts by interacting with mRNAs and cellular export proteins. Probably acts as a viral splicing factor that regulates viral RNA splicing. Functions as a multifunctional regulator of the expression of viral lytic genes (By similarity).
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Kaposi's sarcoma-associated herpesvirus (KSHV) transcribes a long noncoding polyadenylated nuclear (PAN) RNA, which promotes the latent to lytic transition by repressing host genes involved in antiviral responses as well as viral proteins that support the latent state. KSHV also expresses several early proteins including ORF57 (Mta), a member of the conserved multifunctional ICP27 protein family, which is essential for productive replication. ORF57/Mta interacts with PAN RNA via a region termed the Mta responsive element (MRE), stabilizing the transcript and supporting nuclear accumulation. Here, using a close homolog of KSHV ORF57 from herpesvirus saimiri (HVS), we determined the crystal structure of the globular domain in complex with a PAN RNA MRE, revealing a uracil specific binding site that is also conserved in KSHV. Using solution NMR, RNA binding was also mapped within the disordered N-terminal domain of KSHV ORF57, and showed specificity for an RNA fragment containing a GAAGRG motif previously known to bind a homologous region in HVS ORF57. Together these data located novel differential RNA recognition sites within neighboring domains of herpesvirus ORF57 homologs, and revealed high-resolution details of their interactions with PAN RNA, thus providing insight into interactions crucial to viral function.
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Structural identification of conserved RNA binding sites in herpesvirus ORF57 homologs: implications for PAN RNA recognition.,Tunnicliffe RB, Levy C, Ruiz Nivia HD, Sandri-Goldin RM, Golovanov AP Nucleic Acids Res. 2018 Nov 20. pii: 5193347. doi: 10.1093/nar/gky1181. PMID:30462297<ref>PMID:30462297</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6hau" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Herpesvirus saimiri]]
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[[Category: Human gammaherpesvirus 8]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Golovanov, A P]]
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[[Category: Golovanov AP]]
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[[Category: Levy, C]]
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[[Category: Levy C]]
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[[Category: Nivia, H D.Ruiz]]
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[[Category: Ruiz Nivia HD]]
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[[Category: Sandri-Goldin, R M]]
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[[Category: Sandri-Goldin RM]]
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[[Category: Tunnicliffe, R B]]
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[[Category: Tunnicliffe RB]]
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[[Category: Homodimer]]
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[[Category: Icp27]]
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[[Category: Ihd]]
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[[Category: Rna]]
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[[Category: Rna binding protein]]
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Current revision

KSHV PAN RNA Mta-response element fragment complexed with the globular domain of herpesvirus saimiri ORF57

PDB ID 6hau

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