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7z4r

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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[7z4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z4R FirstGlance]. <br>
<table><tr><td colspan='2'>[[7z4r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_falciparum_3D7 Plasmodium falciparum 3D7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Z4R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Z4R FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z4r OCA], [https://pdbe.org/7z4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z4r RCSB], [https://www.ebi.ac.uk/pdbsum/7z4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z4r ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7z4r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7z4r OCA], [https://pdbe.org/7z4r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7z4r RCSB], [https://www.ebi.ac.uk/pdbsum/7z4r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7z4r ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[https://www.uniprot.org/uniprot/C6KTA4_PLAF7 C6KTA4_PLAF7]]
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[https://www.uniprot.org/uniprot/C6KTA4_PLAF7 C6KTA4_PLAF7]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The protozoan parasite Plasmodium falciparum causes the most severe form of malaria and is highly dependent on glycolysis. Glycolytic enzymes were shown to be massively redox regulated, inter alia via oxidative post-translational modifications (oxPTMs) of their cysteine residues. In this study, we identified P. falciparum pyruvate kinase (PfPK) C49 and C343 as amino acid residues essentially involved in maintaining structural and functional integrity of the enzyme. The mutation of these cysteines resulted in an altered substrate affinity, lower enzymatic activities, and, as studied by X-ray crystallography, conformational changes within the A-domain where the substrate binding site is located. Although the loss of a cysteine evoked an impaired catalysis in both mutants, the effects observed for mutant C49A were more severe: multiple conformational changes, caused by the loss of two hydrogen bonds, impeded proper substrate binding and thus the transfer of phosphate upon catalysis.
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Prominent role of cysteine residues C49 and C343 in regulating Plasmodiumfalciparum pyruvate kinase activity.,Dillenberger M, Rahlfs S, Becker K, Fritz-Wolf K Structure. 2022 Aug 17. pii: S0969-2126(22)00316-1. doi:, 10.1016/j.str.2022.08.001. PMID:35998635<ref>PMID:35998635</ref>
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==See Also==
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*[[Pyruvate kinase 3D structures|Pyruvate kinase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7z4r" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>

Current revision

Plasmodium falciparum pyruvate kinase mutant - C343A

PDB ID 7z4r

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