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1rat

From Proteopedia

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(New page: 200px<br /><applet load="1rat" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rat, resolution 1.5&Aring;" /> '''EFFECTS OF TEMPERATUR...)
Current revision (08:21, 1 May 2024) (edit) (undo)
 
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[[Image:1rat.gif|left|200px]]<br /><applet load="1rat" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rat, resolution 1.5&Aring;" />
 
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'''EFFECTS OF TEMPERATURE ON PROTEIN STRUCTURE AND DYNAMICS: X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PROTEIN RIBONUCLEASE-A AT NINE DIFFERENT TEMPERATURES FROM 98 TO 320 K'''<br />
 
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==Overview==
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==EFFECTS OF TEMPERATURE ON PROTEIN STRUCTURE AND DYNAMICS: X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PROTEIN RIBONUCLEASE-A AT NINE DIFFERENT TEMPERATURES FROM 98 TO 320 K==
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Structures using X-ray diffraction data collected to 1.5-A resolution have, been determined for the protein ribonuclease-A at nine different, temperatures ranging from 98 to 320 K. It is determined that the protein, molecule expands slightly (0.4% per 100 K) with increasing temperature and, that this expansion is linear. The expansion is due primarily to subtle, repacking of the molecule, with exposed and mobile loop regions exhibiting, the largest movements. Individual atomic Debye-Waller factors exhibit, predominantly biphasic behavior, with a small positive slope at low, temperatures and a larger positive slope at higher temperatures. The break, in this curve occurs at a characteristic temperature of 180-200 K, perhaps, indicative of fundamental changes in the dynamical structure of the, surrounding protein solvent. The distribution of protein Debye-Waller, factors is observed to broaden as well as shift to higher values as the, temperature is increased.
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<StructureSection load='1rat' size='340' side='right'caption='[[1rat]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RAT FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rat OCA], [https://pdbe.org/1rat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rat RCSB], [https://www.ebi.ac.uk/pdbsum/1rat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rat ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/1rat_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rat ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RAT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RAT OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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Effects of temperature on protein structure and dynamics: X-ray crystallographic studies of the protein ribonuclease-A at nine different temperatures from 98 to 320 K., Tilton RF Jr, Dewan JC, Petsko GA, Biochemistry. 1992 Mar 10;31(9):2469-81. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1547232 1547232]
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dewan JC]]
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[[Category: Dewan, J.C.]]
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[[Category: Petsko GA]]
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[[Category: Petsko, G.A.]]
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[[Category: Tiltonjunior RF]]
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[[Category: Tiltonjunior, R.F.]]
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[[Category: hydrolase (nucleic acid]]
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[[Category: rna)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:25:05 2007''
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Current revision

EFFECTS OF TEMPERATURE ON PROTEIN STRUCTURE AND DYNAMICS: X-RAY CRYSTALLOGRAPHIC STUDIES OF THE PROTEIN RIBONUCLEASE-A AT NINE DIFFERENT TEMPERATURES FROM 98 TO 320 K

PDB ID 1rat

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