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1ras

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(New page: 200px<br /><applet load="1ras" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ras, resolution 1.7&Aring;" /> '''CRYSTAL STRUCTURE OF ...)
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[[Image:1ras.jpg|left|200px]]<br /><applet load="1ras" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ras, resolution 1.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE A'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE A==
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The crystal structure of RNase A chemically modified with the fluorescent, probe, N-[[(iodoacetyl)-amino]ethyl]-5-naphthylamine-1-sulfonic acid, (1,5-IAENS), has been solved and refined to high resolution. It yields, information on the mode of binding, the mobility of a probe commonly used, in spectroscopic studies, and anion binding sites in RNase A. Trigonal, crystals of the fluorescent derivative grown in sodium or cesium chloride, and ammonium sulfate, pH 5.1, were nearly isomorphous with those of a, semisynthetic RNase [DeMel, et al. (1992) J. Biol. Chem. 267, 247-256]., Refinement starting from semisynthetic RNase led to a model with R = 20%, against 1.7-A diffraction data from crystals in ammonium sulfate and, another model with R = 17% against 1.9-A data taken in the presence of 3 M, NaCl. The second model contains three chloride ions: one is at the active, site, and the other two are at molecular interfaces. Otherwise, the two, models are very similar. The fluorophore has very little effect on the, protein conformation. It is found to be covalently attached to the active, site His-12 with the naphthyl group stacked on the imidazole ring of, His-119. It remains largely accessible to solvent and in a polar, environment on the protein surface, even though the fluorescence emission, spectrum is blue shifted as it is in nonpolar solvents.
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<StructureSection load='1ras' size='340' side='right'caption='[[1ras]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ras]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RAS FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AEN:5-(1-SULFONAPHTHYL)-ACETYLAMINO-ETHYLAMINE'>AEN</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ras FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ras OCA], [https://pdbe.org/1ras PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ras RCSB], [https://www.ebi.ac.uk/pdbsum/1ras PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ras ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ra/1ras_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ras ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RAS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with AEN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RAS OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystal structure of a fluorescent derivative of RNase A., Baudet-Nessler S, Jullien M, Crosio MP, Janin J, Biochemistry. 1993 Aug 24;32(33):8457-64. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8357795 8357795]
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Baudet-Nessler S]]
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[[Category: Baudet-Nessler, S.]]
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[[Category: Crosio M-P]]
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[[Category: Crosio, M.P.]]
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[[Category: Janin J]]
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[[Category: Janin, J.]]
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[[Category: Jullien M]]
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[[Category: Jullien, M.]]
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[[Category: AEN]]
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[[Category: hydrolase(nucleic acid]]
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[[Category: rna)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:25:01 2007''
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Current revision

CRYSTAL STRUCTURE OF A FLUORESCENT DERIVATIVE OF RNASE A

PDB ID 1ras

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