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1rbw

From Proteopedia

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(New page: 200px<br /><applet load="1rbw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rbw, resolution 1.69&Aring;" /> '''RIBONUCLEASE A (E.C....)
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[[Image:1rbw.gif|left|200px]]<br /><applet load="1rbw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rbw, resolution 1.69&Aring;" />
 
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'''RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM'''<br />
 
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==Overview==
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==RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM==
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Virtually all studies of the protein-folding reaction add either heat, acid, or a chemical denaturant to an aqueous protein solution in order to, perturb the protein structure. When chemical denaturants are used, very, high concentrations are usually necessary to observe any change in protein, structure. In a solution with such high denaturant concentrations, both, the structure of the protein and the structure of the solvent around the, protein can be altered. X-ray crystallography is the obvious experimental, technique to probe both types of changes. In this paper, we report the, crystal structures of dihydrofolate reductase with urea and of, ribonuclease A with guanidinium chloride. These two classic denaturants, have similar effects on the native structure of the protein. The most, important change that occurs is a reduction in the overall thermal factor., These structures offer a molecular explanation for the reduction in, mobility. Although the reduction is observed only with the native enzyme, in the crystal, a similar decrease in mobility has also been observed in, the unfolded state in solution (Makhatadze G, Privalov PL. 1992. Protein, interactions with urea and guanidinium chloride: A calorimetric study.
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<StructureSection load='1rbw' size='340' side='right'caption='[[1rbw]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rbw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RBW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAI:GUANIDINE'>GAI</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rbw OCA], [https://pdbe.org/1rbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rbw RCSB], [https://www.ebi.ac.uk/pdbsum/1rbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rbw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rb/1rbw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rbw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RBW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus] with GAI as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RBW OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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The effect of denaturants on protein structure., Dunbar J, Yennawar HP, Banerjee S, Luo J, Farber GK, Protein Sci. 1997 Aug;6(8):1727-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9260285 9260285]
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__TOC__
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</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
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[[Category: Pancreatic ribonuclease]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Dunbar JLH]]
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[[Category: Dunbar, J.L.H.]]
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[[Category: Farber GK]]
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[[Category: Farber, G.K.]]
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[[Category: GAI]]
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[[Category: hydrolase (nucleic acid]]
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[[Category: rna)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:26:55 2007''
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Current revision

RIBONUCLEASE A (E.C.3.1.27.5) WITH GUANIDINIUM

PDB ID 1rbw

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