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1rjc

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[[Image:1rjc.gif|left|200px]]
 
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{{Structure
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==Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme==
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|PDB= 1rjc |SIZE=350|CAPTION= <scene name='initialview01'>1rjc</scene>, resolution 1.40&Aring;
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<StructureSection load='1rjc' size='340' side='right'caption='[[1rjc]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
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<table><tr><td colspan='2'>[[1rjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RJC FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rjc OCA], [https://pdbe.org/1rjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rjc RCSB], [https://www.ebi.ac.uk/pdbsum/1rjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rjc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LYSC_CHICK LYSC_CHICK] Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. Has bacteriolytic activity against M.luteus.<ref>PMID:22044478</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rj/1rjc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rjc ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme'''
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==See Also==
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*[[Antibody 3D structures|Antibody 3D structures]]
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*[[Lysozyme 3D structures|Lysozyme 3D structures]]
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==Overview==
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*[[3D structures of non-human antibody|3D structures of non-human antibody]]
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A central paradigm in immunology states that successful generation of high affinity antibodies necessitates an immense primary repertoire of antigen-combining sites. Much of the diversity of this repertoire is provided by varying one antigen binding loop, created by inserting randomly a D (diversity) gene out of a small pool between the V and J genes. It is therefore assumed that any particular D-encoded region surrounded by different V and J regions adopts a different conformation. We have solved the structure of two lysozyme-specific variable domains of heavy-chain antibodies isolated from two strictly unrelated dromedaries. These antibodies recombined identical D gene sequences to different V and J precursors with significant variance in their V(D)J junctions. Despite these large differences, the D-encoded loop segments adopt remarkably identical architectures, thus directing the antibodies toward identical epitopes. Furthermore, a striking convergent maturation process occurred in the V region, adapting both binders for their sub-nanomolar affinity association with lysozyme. Hence, on a structural level, humoral immunity may rely more on well developed maturation and selection systems than on the acquisition of large primary repertoires.
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== References ==
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<references/>
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==About this Structure==
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__TOC__
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1RJC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Camelus_dromedarius Camelus dromedarius] and [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJC OCA].
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</StructureSection>
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==Reference==
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Strong in vivo maturation compensates for structurally restricted H3 loops in antibody repertoires., De Genst E, Silence K, Ghahroudi MA, Decanniere K, Loris R, Kinne J, Wyns L, Muyldermans S, J Biol Chem. 2005 Apr 8;280(14):14114-21. Epub 2005 Jan 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15659390 15659390]
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[[Category: Camelus dromedarius]]
[[Category: Camelus dromedarius]]
[[Category: Gallus gallus]]
[[Category: Gallus gallus]]
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[[Category: Lysozyme]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: De Genst E]]
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[[Category: Decanniere, K.]]
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[[Category: Decanniere K]]
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[[Category: Genst, E De.]]
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[[Category: Ghahroudi MA]]
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[[Category: Ghahroudi, M A.]]
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[[Category: Kinne J]]
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[[Category: Kinne, J.]]
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[[Category: Loris R]]
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[[Category: Loris, R.]]
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[[Category: Muyldermans S]]
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[[Category: Muyldermans, S.]]
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[[Category: Silence K]]
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[[Category: Silence, K.]]
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[[Category: Wyns L]]
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[[Category: Wyns, L.]]
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[[Category: GOL]]
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[[Category: PO4]]
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[[Category: alpha-beta orthogonal bundle]]
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[[Category: beta sandwich]]
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[[Category: immunoglobulin fold]]
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[[Category: protein-protein hetero complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:52:23 2008''
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Current revision

Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme

PDB ID 1rjc

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