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1rlc

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(New page: 200px<br /> <applet load="1rlc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rlc, resolution 2.7&Aring;" /> '''CRYSTAL STRUCTURE OF...)
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[[Image:1rlc.gif|left|200px]]<br />
 
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<applet load="1rlc" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rlc, resolution 2.7&Aring;" />
 
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'''CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE'''<br />
 
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==Overview==
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==CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE==
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The crystal structure of unactivated ribulose 1,5-bisphosphate, carboxylase/oxygenase from Nicotiana tabacum complexed with a transition, state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate, was determined to, 2.7 A resolution by X-ray crystallography. The transition state analog, binds at the active site in an extended conformation. As compared to the, binding of the same analog in the activated enzyme, the analog binds in a, reverse orientation. The active site Lys 201 is within hydrogen bonding, distance of the carboxyl oxygen of the analog. Loop 6 (residues 330-339), remains open and flexible upon binding of the analog in the unactivated, enzyme, in contrast to the closed and ordered loop 6 in the activated, enzyme complex. The transition state analog is exposed to solvent due to, the open conformation of loop 6.
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<StructureSection load='1rlc' size='340' side='right'caption='[[1rlc]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rlc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. The November 2000 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Rubisco'' by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2000_11 10.2210/rcsb_pdb/mom_2000_11]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RLC FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CAP:2-CARBOXYARABINITOL-1,5-DIPHOSPHATE'>CAP</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rlc OCA], [https://pdbe.org/1rlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rlc RCSB], [https://www.ebi.ac.uk/pdbsum/1rlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rlc ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RBL_TOBAC RBL_TOBAC] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rl/1rlc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rlc ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RLC is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] with CAP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1RLC with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb11_1.html Rubisco]]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RLC OCA].
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*[[RuBisCO 3D structures|RuBisCO 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystal structure of the unactivated ribulose 1,5-bisphosphate carboxylase/oxygenase complexed with a transition state analog, 2-carboxy-D-arabinitol 1,5-bisphosphate., Zhang KY, Cascio D, Eisenberg D, Protein Sci. 1994 Jan;3(1):64-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8142899 8142899]
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[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
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[[Category: Protein complex]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: Ribulose-bisphosphate carboxylase]]
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[[Category: Rubisco]]
[[Category: Rubisco]]
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[[Category: Cascio, D.]]
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[[Category: Cascio D]]
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[[Category: Eisenberg, D.]]
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[[Category: Eisenberg D]]
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[[Category: Zhang, K.Y.J.]]
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[[Category: Zhang KYJ]]
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[[Category: CAP]]
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[[Category: lyase(carbon-carbon)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:05:32 2007''
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Current revision

CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE

PDB ID 1rlc

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