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1rmf

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(New page: 200px<br /> <applet load="1rmf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rmf, resolution 2.8&Aring;" /> '''STRUCTURES OF A MONO...)
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[[Image:1rmf.gif|left|200px]]<br />
 
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<applet load="1rmf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rmf, resolution 2.8&Aring;" />
 
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'''STRUCTURES OF A MONOCLONAL ANTI-ICAM-1 ANTIBODY R6.5 FRAGMENT AT 2.8 ANGSTROMS RESOLUTION'''<br />
 
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==Overview==
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==STRUCTURES OF A MONOCLONAL ANTI-ICAM-1 ANTIBODY R6.5 FRAGMENT AT 2.8 ANGSTROMS RESOLUTION==
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The specific binding of the monoclonal murine anti-intercellular adhesion, molecule-1 (anti-ICAM-1) antibody, R6.5, inhibits the attachment of, neutrophils to endothelium and prevents the attachment of major group, human rhinovirus (HRV) to ICAM-1. This binding interferes with the host, immune system and, as a result, the R6.5 antibody has been developed as a, therapeutic anti-inflammatory and perhaps anti-HRV agent. The, variable-region amino-acid sequence of R6.5 was determined from the, anti-ICAM-1 cDNA. The crystallization conditions of the Fab fragment of, R6.5 were established and the three-dimensional structure was determined, by X-ray crystallography. The crystal space group is orthorhombic, P2(1)2(1)2(1), a = 40.36, b = 137.76, c = 91.32 A, and the highest, resolution of recorded reflections is 2.7 A. The molecular-replacement, method using known Fab structures was employed to solve the R6.5 Fab, structure. The final R-factor is 18.8% for a total of 3320 non-H protein, atoms, 39 water molecules and 10 606 unique reflections. The protein, exhibits the typical immunoglobulin fold. The surface contour of the, antigen-combining site of the R6.5 antibody has a wide groove which, resembles more the structure of an anti-polypeptide antibody than the, structure of an anti-protein antibody.
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<StructureSection load='1rmf' size='340' side='right'caption='[[1rmf]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rmf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmf OCA], [https://pdbe.org/1rmf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmf RCSB], [https://www.ebi.ac.uk/pdbsum/1rmf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmf ProSAT]</span></td></tr>
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</table>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rm/1rmf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rmf ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RMF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RMF OCA].
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*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Structure of a monoclonal anti-ICAM-1 antibody R6.5 Fab fragment at 2.8 A resolution., Jedrzejas MJ, Miglietta J, Griffin JA, Luo M, Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):380-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299305 15299305]
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[[Category: Large Structures]]
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[[Category: Protein complex]]
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[[Category: Mus musculus]]
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[[Category: Jedrzejas, M.J.]]
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[[Category: Jedrzejas MJ]]
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[[Category: Luo, M.]]
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[[Category: Luo M]]
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[[Category: immunoglobulin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:41:32 2007''
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Current revision

STRUCTURES OF A MONOCLONAL ANTI-ICAM-1 ANTIBODY R6.5 FRAGMENT AT 2.8 ANGSTROMS RESOLUTION

PDB ID 1rmf

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