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1rmj

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(New page: 200px<br /> <applet load="1rmj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rmj" /> '''C-terminal domain of insulin-like growth fa...)
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[[Image:1rmj.gif|left|200px]]<br />
 
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<applet load="1rmj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rmj" />
 
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'''C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II'''<br />
 
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==Overview==
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==C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II==
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IGFs are important mediators of growth. IGF binding proteins (IGFBPs) 1-6, regulate IGF actions and have IGF-independent actions. The C-terminal, domains of IGFBPs contribute to high-affinity IGF binding and modulation, of IGF actions and confer some IGF-independent properties, but, understanding how they achieve this has been constrained by the lack of a, three-dimensional structure. We therefore determined the solution, structure of the C-domain of IGFBP-6 using nuclear magnetic resonance, (NMR). The domain consists of a thyroglobulin type 1 fold comprising an, alpha-helix followed by a loop, a three-stranded antiparallel beta-sheet, incorporating a second loop, and finally a disulfide-bonded flexible third, loop. The IGF-II binding site on the C-domain was identified by examining, NMR spectral changes upon complex formation. It consists of a largely, hydrophobic surface patch involving the alpha-helix, the first, beta-strand, and the first and second loops. The site was confirmed by, mutagenesis of several residues, which resulted in decreased IGF binding, affinity. The IGF-II binding site lies adjacent to surfaces likely to be, involved in glycosaminoglycan binding of IGFBPs, which might explain their, decreased IGF affinity when bound to glycosaminoglycans, and nuclear, localization. Our structure provides a framework for understanding the, roles of IGFBP C-domains in modulating IGF actions and conferring, IGF-independent actions, as well as ultimately for the development of, therapeutic IGF inhibitors for diseases including cancer.
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<StructureSection load='1rmj' size='340' side='right'caption='[[1rmj]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rmj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RMJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RMJ FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rmj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rmj OCA], [https://pdbe.org/1rmj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rmj RCSB], [https://www.ebi.ac.uk/pdbsum/1rmj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rmj ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/IBP6_HUMAN IBP6_HUMAN] IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rm/1rmj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rmj ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RMJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RMJ OCA].
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*[[Insulin-like growth factor binding protein 3D structures|Insulin-like growth factor binding protein 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II., Headey SJ, Keizer DW, Yao S, Brasier G, Kantharidis P, Bach LA, Norton RS, Mol Endocrinol. 2004 Nov;18(11):2740-50. Epub 2004 Aug 12. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15308688 15308688]
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Bach, L.A.]]
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[[Category: Bach LA]]
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[[Category: Brasier, G.]]
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[[Category: Brasier G]]
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[[Category: Headey, S.J.]]
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[[Category: Headey SJ]]
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[[Category: Kantharidis, P.]]
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[[Category: Kantharidis P]]
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[[Category: Keizer, D.W.]]
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[[Category: Keizer DW]]
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[[Category: Norton, R.S.]]
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[[Category: Norton RS]]
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[[Category: Yao, S.]]
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[[Category: Yao S]]
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[[Category: insulin-like growth factor binding protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:05:31 2007''
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Current revision

C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II

PDB ID 1rmj

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