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1rne

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(New page: 200px<br /> <applet load="1rne" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rne, resolution 2.4&Aring;" /> '''THE CRYSTAL STRUCTUR...)
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[[Image:1rne.gif|left|200px]]<br />
 
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<applet load="1rne" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rne, resolution 2.4&Aring;" />
 
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'''THE CRYSTAL STRUCTURE OF RECOMBINANT GLYCOSYLATED HUMAN RENIN ALONE AND IN COMPLEX WITH A TRANSITION STATE ANALOG INHIBITOR'''<br />
 
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==Overview==
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==THE CRYSTAL STRUCTURE OF RECOMBINANT GLYCOSYLATED HUMAN RENIN ALONE AND IN COMPLEX WITH A TRANSITION STATE ANALOG INHIBITOR==
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Recombinant human glycosylated renin has been crystallized in complex with, CGP 38'560, a transition state analog inhibitor (IC50 = 2 x 10(-9) M), in, a tetragonal crystal form. The structure has been determined to a, resolution of 2.4 A and refined to a crystallographic Rfactor of 17.6%. It, reveals the conformation of the inhibitor as well as its interactions with, the enzyme active site. The active site is a deep cleft between the N- and, the C-terminal domains to which the inhibitor binds in an extended, conformation filling the S4 to S2' pockets. The structure of the complex, is compared with that of the related uninhibited enzyme pepsin., Significant changes in the relative orientation of the N- and C-terminal, domains are observed. In the inhibited renin structure the C-terminal loop, segments forming the active site are closer to those from the N-terminal, domain than in the related "open" pepsin structure. In addition, the, structure of uninhibited glycosylated renin has been determined at 2.8 A, resolution from a cubic crystal form with two renin molecules in the, asymmetric unit. The two independent renin molecules show different, conformations with respect to the relative orientation of their N- and, C-terminal domains; one molecule is found in the "closed inhibited", conformation, the other in the "open uninhibited" conformation.
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<StructureSection load='1rne' size='340' side='right'caption='[[1rne]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1rne]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RNE FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C60:[[[3-(2-METHYL-PROPANE-2-SULFONYL)-1-BENZENYL]-2-PROPYL]-CARBONYL-HISTIDYL]-AMINO-[CYCLOHEXYLMETHYL]-[2-HYDROXY-4-ISOPROPYL]-PENTAN-5-OIC+ACID+BUTYLAMIDE'>C60</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rne FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rne OCA], [https://pdbe.org/1rne PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rne RCSB], [https://www.ebi.ac.uk/pdbsum/1rne PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rne ProSAT]</span></td></tr>
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</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/RENI_HUMAN RENI_HUMAN] Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:[https://omim.org/entry/267430 267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).<ref>PMID:16116425</ref> Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:[https://omim.org/entry/613092 613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia.<ref>PMID:19664745</ref>
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== Function ==
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[https://www.uniprot.org/uniprot/RENI_HUMAN RENI_HUMAN] Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/1rne_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rne ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1RNE is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with NGA and C60 as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Renin Renin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.23.15 3.4.23.15] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RNE OCA].
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*[[Renin|Renin]]
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== References ==
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==Reference==
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<references/>
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The crystal structures of recombinant glycosylated human renin alone and in complex with a transition state analog inhibitor., Rahuel J, Priestle JP, Grutter MG, J Struct Biol. 1991 Dec;107(3):227-36. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1807356 1807356]
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Renin]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Gruetter MG]]
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[[Category: Gruetter, M.G.]]
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[[Category: Priestle JP]]
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[[Category: Priestle, J.P.]]
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[[Category: Rahuel J]]
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[[Category: Rahuel, J.]]
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[[Category: C60]]
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[[Category: NGA]]
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[[Category: hydrolase(acid proteinase)]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 12:43:19 2007''
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Current revision

THE CRYSTAL STRUCTURE OF RECOMBINANT GLYCOSYLATED HUMAN RENIN ALONE AND IN COMPLEX WITH A TRANSITION STATE ANALOG INHIBITOR

PDB ID 1rne

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