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1rni

From Proteopedia

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[[Image:1rni.jpg|left|200px]]
 
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{{Structure
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==Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1==
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|PDB= 1rni |SIZE=350|CAPTION= <scene name='initialview01'>1rni</scene>, resolution 1.85&Aring;
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<StructureSection load='1rni' size='340' side='right'caption='[[1rni]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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<table><tr><td colspan='2'>[[1rni]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RNI FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rni OCA], [https://pdbe.org/1rni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rni RCSB], [https://www.ebi.ac.uk/pdbsum/1rni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rni ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1ro0|1RO0]], [[1ro2|1RO2]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rni OCA], [http://www.ebi.ac.uk/pdbsum/1rni PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1rni RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q54324_SULIS Q54324_SULIS]
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== Evolutionary Conservation ==
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'''Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1'''
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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==Overview==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/1rni_consurf.spt"</scriptWhenChecked>
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Genome replication generally requires primases, which synthesize an initial oligonucleotide primer, and DNA polymerases, which elongate the primer. Primase and DNA polymerase activities are combined, however, in newly identified replicases from archaeal plasmids, such as pRN1 from Sulfolobus islandicus. Here we present a structure-function analysis of the pRN1 primase-polymerase (prim-pol) domain. The crystal structure shows a central depression lined by conserved residues. Mutations on one side of the depression reduce DNA affinity. On the opposite side of the depression cluster three acidic residues and a histidine, which are required for primase and DNA polymerase activity. One acidic residue binds a manganese ion, suggestive of a metal-dependent catalytic mechanism. The structure does not show any similarity to DNA polymerases, but is distantly related to archaeal and eukaryotic primases, with corresponding active-site residues. We propose that archaeal and eukaryotic primases and the prim-pol domain have a common evolutionary ancestor, a bifunctional replicase for small DNA genomes.
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==About this Structure==
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</jmolCheckbox>
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1RNI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNI OCA].
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rni ConSurf].
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<div style="clear:both"></div>
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==Reference==
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__TOC__
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Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14730355 14730355]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Large Structures]]
[[Category: Sulfolobus islandicus]]
[[Category: Sulfolobus islandicus]]
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[[Category: Buchen, C.]]
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[[Category: Buchen C]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Lipps, G.]]
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[[Category: Lipps G]]
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[[Category: Scheven, G von.]]
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[[Category: Weinzierl AO]]
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[[Category: Weinzierl, A O.]]
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[[Category: Von Scheven G]]
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[[Category: dna polymerase]]
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[[Category: evolution of nucleic acid polymerizing enzyme]]
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[[Category: polymerization]]
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[[Category: primase]]
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[[Category: replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:30:32 2008''
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Current revision

Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1

PDB ID 1rni

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