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1rni

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(New page: 200px<br /><applet load="1rni" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rni, resolution 1.85&Aring;" /> '''Bifunctional DNA pri...)
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[[Image:1rni.jpg|left|200px]]<br /><applet load="1rni" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rni, resolution 1.85&Aring;" />
 
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'''Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1'''<br />
 
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==Overview==
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==Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1==
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Genome replication generally requires primases, which synthesize an, initial oligonucleotide primer, and DNA polymerases, which elongate the, primer. Primase and DNA polymerase activities are combined, however, in, newly identified replicases from archaeal plasmids, such as pRN1 from, Sulfolobus islandicus. Here we present a structure-function analysis of, the pRN1 primase-polymerase (prim-pol) domain. The crystal structure shows, a central depression lined by conserved residues. Mutations on one side of, the depression reduce DNA affinity. On the opposite side of the depression, cluster three acidic residues and a histidine, which are required for, primase and DNA polymerase activity. One acidic residue binds a manganese, ion, suggestive of a metal-dependent catalytic mechanism. The structure, does not show any similarity to DNA polymerases, but is distantly related, to archaeal and eukaryotic primases, with corresponding active-site, residues. We propose that archaeal and eukaryotic primases and the, prim-pol domain have a common evolutionary ancestor, a bifunctional, replicase for small DNA genomes.
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<StructureSection load='1rni' size='340' side='right'caption='[[1rni]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rni]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RNI FirstGlance]. <br>
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1RNI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_islandicus Sulfolobus islandicus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RNI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rni FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rni OCA], [https://pdbe.org/1rni PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rni RCSB], [https://www.ebi.ac.uk/pdbsum/1rni PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rni ProSAT]</span></td></tr>
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Structure of a bifunctional DNA primase-polymerase., Lipps G, Weinzierl AO, von Scheven G, Buchen C, Cramer P, Nat Struct Mol Biol. 2004 Feb;11(2):157-62. Epub 2004 Jan 18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14730355 14730355]
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</table>
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[[Category: Single protein]]
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== Function ==
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[https://www.uniprot.org/uniprot/Q54324_SULIS Q54324_SULIS]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rn/1rni_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rni ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Sulfolobus islandicus]]
[[Category: Sulfolobus islandicus]]
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[[Category: Buchen, C.]]
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[[Category: Buchen C]]
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[[Category: Cramer, P.]]
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[[Category: Cramer P]]
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[[Category: Lipps, G.]]
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[[Category: Lipps G]]
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[[Category: Scheven, G.von.]]
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[[Category: Weinzierl AO]]
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[[Category: Weinzierl, A.O.]]
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[[Category: Von Scheven G]]
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[[Category: ZN]]
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[[Category: dna polymerase]]
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[[Category: evolution of nucleic acid polymerizing enzymes]]
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[[Category: polymerization]]
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[[Category: primase]]
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[[Category: replication]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 22:58:49 2007''
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Current revision

Bifunctional DNA primase/polymerase domain of ORF904 from the archaeal plasmid pRN1

PDB ID 1rni

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