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1rwj

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(New page: 200px<br /><applet load="1rwj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rwj, resolution 1.70&Aring;" /> '''c7-type three-heme c...)
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[[Image:1rwj.gif|left|200px]]<br /><applet load="1rwj" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rwj, resolution 1.70&Aring;" />
 
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'''c7-type three-heme cytochrome domain'''<br />
 
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==Overview==
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==c7-type three-heme cytochrome domain==
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The structure of a novel c(7)-type cytochrome domain that has two, bishistidine coordinated hemes and one heme with histidine, methionine, coordination (where the sixth ligand is a methionine residue) was, determined at 1.7 A resolution. This domain is a representative of domains, that form three polymers encoded by the Geobacter sulfurreducens genome., Two of these polymers consist of four and one protein of nine c(7)-type, domains with a total of 12 and 27 hemes, respectively. Four individual, domains (termed A, B, C, and D) from one such multiheme cytochrome c, (ORF03300) were cloned and expressed in Escherichia coli. The domain C, produced diffraction quality crystals from 2.4 M sodium malonate (pH 7)., The structure was solved by MAD method and refined to an R-factor of 19.5%, and R-free of 21.8%. Unlike the two c(7) molecules with known structures, one from G. sulfurreducens (PpcA) and one from Desulfuromonas acetoxidans, where all three hemes are bishistidine coordinated, this domain contains a, heme which is coordinated by a methionine and a histidine residue. As a, result, the corresponding heme could have a higher potential than the, other two hemes. The apparent midpoint reduction potential, E(app), of, domain C is -105 mV, 50 mV higher than that of PpcA.
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<StructureSection load='1rwj' size='340' side='right'caption='[[1rwj]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rwj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RWJ FirstGlance]. <br>
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1RWJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Geobacter_sulfurreducens Geobacter sulfurreducens] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RWJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rwj OCA], [https://pdbe.org/1rwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rwj RCSB], [https://www.ebi.ac.uk/pdbsum/1rwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rwj ProSAT]</span></td></tr>
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Structure of a novel c7-type three-heme cytochrome domain from a multidomain cytochrome c polymer., Pokkuluri PR, Londer YY, Duke NE, Erickson J, Pessanha M, Salgueiro CA, Schiffer M, Protein Sci. 2004 Jun;13(6):1684-92. Epub 2004 May 7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15133162 15133162]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q74BP5_GEOSL Q74BP5_GEOSL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rw/1rwj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rwj ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Geobacter sulfurreducens]]
[[Category: Geobacter sulfurreducens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Duke, N.E.C.]]
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[[Category: Duke NEC]]
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[[Category: Erickson, J.]]
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[[Category: Erickson J]]
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[[Category: Londer, Y.Y.]]
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[[Category: Londer YY]]
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[[Category: Pessanha, M.]]
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[[Category: Pessanha M]]
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[[Category: Pokkuluri, P.R.]]
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[[Category: Pokkuluri PR]]
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[[Category: Salgueiro, C.A.]]
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[[Category: Salgueiro CA]]
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[[Category: Schiffer, M.]]
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[[Category: Schiffer M]]
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[[Category: HEM]]
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[[Category: cytochrome c7]]
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[[Category: geobacter metallireducens]]
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[[Category: geobacter sulfurreducens]]
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[[Category: heme coordination in c-type cytochromes]]
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[[Category: multiheme cytochrome c]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sat Nov 24 23:25:59 2007''
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Current revision

c7-type three-heme cytochrome domain

PDB ID 1rwj

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