This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1rxo

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:27, 1 May 2024) (edit) (undo)
 
(21 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1rxo.gif|left|200px]]<br />
 
-
<applet load="1rxo" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1rxo, resolution 2.2&Aring;" />
 
-
'''ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE AND CALCIUM'''<br />
 
-
==Overview==
+
==ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE AND CALCIUM==
-
The three-dimensional structure of the complex of ribulose, 1,5-bisphosphate carboxylase/oxygenase (rubisco; EC 4.1.1.39) from spinach, with its natural substrate ribulose 1,5-bisphosphate (RuBP) has been, determined both under activating and non-activating conditions by X-ray, crystallography to a resolution of 2.1 A and 2.4 A, respectively. Under, activating conditions, the use of calcium instead of magnesium as the, activator metal ion enabled us to trap the substrate in a stable complex, for crystallographic analysis. Comparison of the structure of the, activated and the non-activated RuBP complexes shows a tighter binding for, the substrate in the non-activated form of the enzyme, in line with, previous solution studies. In the non-activated complex, the substrate, triggers isolation ... [[http://ispc.weizmann.ac.il/pmbin/getpm?9034362 (full description)]]
+
<StructureSection load='1rxo' size='340' side='right'caption='[[1rxo]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1rxo]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RXO FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
 +
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene>, <scene name='pdbligand=RUB:RIBULOSE-1,5-DIPHOSPHATE'>RUB</scene></td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rxo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rxo OCA], [https://pdbe.org/1rxo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rxo RCSB], [https://www.ebi.ac.uk/pdbsum/1rxo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rxo ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/RBL_SPIOL RBL_SPIOL] RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.[HAMAP-Rule:MF_01338]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rx/1rxo_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rxo ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1RXO is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]] with RUB and CA as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39]]. Structure known Active Sites: ACB, ACE, ACH and ACL. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RXO OCA]].
+
*[[RuBisCO 3D structures|RuBisCO 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
The structure of the complex between rubisco and its natural substrate ribulose 1,5-bisphosphate., Taylor TC, Andersson I, J Mol Biol. 1997 Jan 31;265(4):432-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9034362 9034362]
+
[[Category: Large Structures]]
-
[[Category: Protein complex]]
+
-
[[Category: Ribulose-bisphosphate carboxylase]]
+
[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
-
[[Category: Andersson, I.]]
+
[[Category: Andersson I]]
-
[[Category: Taylor, T.C.]]
+
[[Category: Taylor TC]]
-
[[Category: CA]]
+
-
[[Category: RUB]]
+
-
[[Category: lyase (carbon-carbon)]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:22:22 2007''
+

Current revision

ACTIVATED SPINACH RUBISCO IN COMPLEX WITH ITS SUBSTRATE RIBULOSE-1,5-BISPHOSPHATE AND CALCIUM

PDB ID 1rxo

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1rxo"
Personal tools