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1rxq

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[[Image:1rxq.gif|left|200px]]
 
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{{Structure
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==YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology==
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|PDB= 1rxq |SIZE=350|CAPTION= <scene name='initialview01'>1rxq</scene>, resolution 1.70&Aring;
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<StructureSection load='1rxq' size='340' side='right'caption='[[1rxq]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> and <scene name='pdbligand=GLU:GLUTAMIC ACID'>GLU</scene>
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<table><tr><td colspan='2'>[[1rxq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RXQ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
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|GENE= yfiT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ALA:ALANINE'>ALA</scene>, <scene name='pdbligand=GLU:GLUTAMIC+ACID'>GLU</scene>, <scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SER:SERINE'>SER</scene>, <scene name='pdbligand=THR:THREONINE'>THR</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rxq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rxq OCA], [https://pdbe.org/1rxq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rxq RCSB], [https://www.ebi.ac.uk/pdbsum/1rxq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rxq ProSAT], [https://www.topsan.org/Proteins/MCSG/1rxq TOPSAN]</span></td></tr>
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</table>
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'''YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology'''
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== Function ==
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[https://www.uniprot.org/uniprot/YFIT_BACSU YFIT_BACSU] Possible metal-dependent hydrolase.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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YfiT, a 19-kDa polypeptide from Bacillus subtilis, belongs to a small sequence family with members predominantly from Gram positive bacteria. We have determined the crystal structure of YfiT in complex with Ni(2+) to a resolution of 1.7 A. YfiT exists as a dimer and binds Ni(2+) in a 1:1 stoichiometry. The protein has an unusual four-helix bundle topology and coordinates Ni(2+) in an octahedral geometry with three conserved histidines and three waters. Although there is no similarity in their overall structures, the coordination geometry of the metal and the residues that constitute the putative active site in YfiT are similar to those of metalloproteases such as thermolysin. Our structural analyses suggest that YfiT might function as a metal-dependent hydrolase.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rx/1rxq_consurf.spt"</scriptWhenChecked>
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1RXQ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RXQ OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology., Rajan SS, Yang X, Shuvalova L, Collart F, Anderson WF, Biochemistry. 2004 Dec 14;43(49):15472-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15581359 15581359]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rxq ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Anderson, W F.]]
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[[Category: Anderson WF]]
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[[Category: MCSG, Midwest Center for Structural Genomics.]]
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[[Category: Rajan SS]]
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[[Category: Rajan, S S.]]
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[[Category: Yang X]]
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[[Category: Yang, X.]]
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[[Category: GLU]]
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[[Category: NI]]
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[[Category: helix-bundle]]
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[[Category: hydrolase]]
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[[Category: mcsg]]
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[[Category: midwest center for structural genomic]]
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[[Category: nickel-binding]]
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[[Category: protein structure initiative]]
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[[Category: psi]]
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[[Category: structural genomic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:57:48 2008''
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Current revision

YfiT from Bacillus subtilis is a probable metal-dependent hydrolase with an unusual four-helix bundle topology

PDB ID 1rxq

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