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1rzv

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(New page: 200px<br /><applet load="1rzv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rzv, resolution 2.30&Aring;" /> '''Crystal structure of...)
Current revision (08:28, 1 May 2024) (edit) (undo)
 
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[[Image:1rzv.jpg|left|200px]]<br /><applet load="1rzv" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1rzv, resolution 2.30&Aring;" />
 
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'''Crystal structure of the glycogen synthase from Agrobacterium tumefaciens (non-complexed form)'''<br />
 
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==Overview==
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==Crystal structure of the glycogen synthase from Agrobacterium tumefaciens (non-complexed form)==
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Glycogen and starch are the major readily accessible energy storage, compounds in nearly all living organisms. Glycogen is a very large, branched glucose homopolymer containing about 90% alpha-1,4-glucosidic, linkages and 10% alpha-1,6 linkages. Its synthesis and degradation, constitute central pathways in the metabolism of living cells regulating a, global carbon/energy buffer compartment. Glycogen biosynthesis involves, the action of several enzymes among which glycogen synthase catalyzes the, synthesis of the alpha-1,4-glucose backbone. We now report the first, crystal structure of glycogen synthase in the presence and absence of, adenosine diphosphate. The overall fold and the active site architecture, of the protein are remarkably similar to those of glycogen phosphorylase, indicating a common catalytic mechanism and comparable substrate-binding, properties. In contrast to glycogen phosphorylase, glycogen synthase has a, much wider catalytic cleft, which is predicted to undergo an important, interdomain 'closure' movement during the catalytic cycle. The structures, also provide useful hints to shed light on the allosteric regulation, mechanisms of yeast/mammalian glycogen synthases.
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<StructureSection load='1rzv' size='340' side='right'caption='[[1rzv]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1rzv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZV FirstGlance]. <br>
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1RZV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Agrobacterium_tumefaciens Agrobacterium tumefaciens]. Active as [http://en.wikipedia.org/wiki/Starch_synthase Starch synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.21 2.4.1.21] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RZV OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rzv OCA], [https://pdbe.org/1rzv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rzv RCSB], [https://www.ebi.ac.uk/pdbsum/1rzv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rzv ProSAT]</span></td></tr>
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Crystal structure of glycogen synthase: homologous enzymes catalyze glycogen synthesis and degradation., Buschiazzo A, Ugalde JE, Guerin ME, Shepard W, Ugalde RA, Alzari PM, EMBO J. 2004 Aug 18;23(16):3196-205. Epub 2004 Jul 22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15272305 15272305]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/GLGA1_RHIRD GLGA1_RHIRD] Synthesizes alpha-1,4-glucan chains using ADP-glucose.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rzv_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rzv ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Agrobacterium tumefaciens]]
[[Category: Agrobacterium tumefaciens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Starch synthase]]
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[[Category: Alzari PM]]
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[[Category: Alzari, P.M.]]
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[[Category: Buschiazzo A]]
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[[Category: Buschiazzo, A.]]
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[[Category: Guerin ME]]
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[[Category: Guerin, M.E.]]
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[[Category: Shepard W]]
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[[Category: Shepard, W.]]
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[[Category: Ugalde JE]]
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[[Category: Ugalde, J.E.]]
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[[Category: Ugalde RA]]
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[[Category: Ugalde, R.A.]]
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[[Category: glycosyl-transferase gt-b fold]]
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[[Category: rossman fold]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:59:31 2007''
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Current revision

Crystal structure of the glycogen synthase from Agrobacterium tumefaciens (non-complexed form)

PDB ID 1rzv

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