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1s4i

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[[Image:1s4i.gif|left|200px]]
 
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{{Structure
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==Crystal structure of a SOD-like protein from Bacillus subtilis==
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|PDB= 1s4i |SIZE=350|CAPTION= <scene name='initialview01'>1s4i</scene>, resolution 1.80&Aring;
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<StructureSection load='1s4i' size='340' side='right'caption='[[1s4i]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=CL:CHLORIDE ION'>CL</scene>
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<table><tr><td colspan='2'>[[1s4i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S4I FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Superoxide_dismutase Superoxide dismutase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.15.1.1 1.15.1.1]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
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|GENE= YOJM, BSU19400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4i OCA], [https://pdbe.org/1s4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s4i RCSB], [https://www.ebi.ac.uk/pdbsum/1s4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4i ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of a SOD-like protein from Bacillus subtilis'''
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== Function ==
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[https://www.uniprot.org/uniprot/YOJM_BACSU YOJM_BACSU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Little is known about prokaryotic homologs of Cu,Zn superoxide dismutase (SOD), an enzyme highly conserved among eukaryotic species. In 138 Archaea and Bacteria genomes, 57 of these putative homologs were found, 11 of which lack at least one of the metal ligands. Both the solution and the crystal structures of the SOD-like protein from Bacillus subtilis, lacking two Cu ligands and found to be enzymatically inactive, were determined. In solution, the protein is monomeric. The available nuclear Overhauser effects, together with chemical-shift index values, allowed us to define and to recognize the typical Cu,Zn SOD Greek beta-barrel but with largely unstructured loops (which, therefore, sample a wide range of conformations). On the contrary, in the crystal structure (obtained in the presence of slight excess of Zn), the protein is well structured and organized in covalent dimers held by a symmetric bridge consisting of a Zn ion bound to an Asp-His dyad in a tetrahedral geometry. Couples of dimers held by hydrophobic interactions and H bonds are further organized in long chains. The order/disorder transition is discussed in terms of metal binding and physical state.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4i_consurf.spt"</scriptWhenChecked>
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1S4I is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4I OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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A prokaryotic superoxide dismutase paralog lacking two Cu ligands: from largely unstructured in solution to ordered in the crystal., Banci L, Bertini I, Calderone V, Cramaro F, Del Conte R, Fantoni A, Mangani S, Quattrone A, Viezzoli MS, Proc Natl Acad Sci U S A. 2005 May 24;102(21):7541-6. Epub 2005 May 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15897454 15897454]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4i ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Superoxide dismutase]]
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[[Category: Banci L]]
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[[Category: Banci, L.]]
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[[Category: Bertini I]]
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[[Category: Bertini, I.]]
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[[Category: Calderone V]]
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[[Category: Calderone, V.]]
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[[Category: Cramaro F]]
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[[Category: Conte, R Del.]]
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[[Category: Del Conte R]]
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[[Category: Cramaro, F.]]
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[[Category: Fantoni A]]
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[[Category: Fantoni, A.]]
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[[Category: Mangani S]]
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[[Category: Mangani, S.]]
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[[Category: Quattrone A]]
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[[Category: Quattrone, A.]]
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[[Category: Viezzoli MS]]
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[[Category: Viezzoli, M S.]]
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[[Category: CL]]
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[[Category: ZN]]
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[[Category: cu-zn sod]]
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[[Category: sod]]
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[[Category: sod-like]]
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[[Category: superoxide dismutase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:00:18 2008''
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Current revision

Crystal structure of a SOD-like protein from Bacillus subtilis

PDB ID 1s4i

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