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1s5y

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[[Image:1s5y.jpg|left|200px]]
 
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{{Structure
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==The crystal structure of Trematomus bernacchii hemoglobin oxidized by ferricyanide==
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|PDB= 1s5y |SIZE=350|CAPTION= <scene name='initialview01'>1s5y</scene>, resolution 2.50&Aring;
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<StructureSection load='1s5y' size='340' side='right'caption='[[1s5y]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>
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<table><tr><td colspan='2'>[[1s5y]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S5Y FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5y OCA], [https://pdbe.org/1s5y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s5y RCSB], [https://www.ebi.ac.uk/pdbsum/1s5y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s5y ProSAT]</span></td></tr>
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|RELATEDENTRY=[[1s5x|1S5X]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s5y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s5y OCA], [http://www.ebi.ac.uk/pdbsum/1s5y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1s5y RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/HBA_TREBE HBA_TREBE] Involved in oxygen transport from gills to the various peripheral tissues.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s5/1s5y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s5y ConSurf].
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<div style="clear:both"></div>
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'''The crystal structure of Trematomus bernacchii hemoglobin oxidized by ferricyanide'''
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==See Also==
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*[[Hemoglobin 3D structures|Hemoglobin 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Analysis of the molecular properties of proteins extracted from organisms living under extreme conditions often highlights peculiar features. We investigated by UV-visible spectroscopy and X-ray crystallography the oxidation process, promoted by air or ferricyanide, of five hemoglobins extracted from Antarctic fishes (Notothenioidei). Spectroscopic analysis revealed that these hemoglobins share a common oxidation pathway, which shows striking differences from the oxidation processes of hemoglobins from other vertebrates. Indeed, simple exposure of these hemoglobins to air leads to the formation of a significant amount of the low-spin hexacoordinated form, denoted hemichrome. This hemichrome form, which is detected under a variety of experimental conditions, can be reversibly transformed to either carbomonoxy or deoxygenated forms with reducing agents. Interestingly, the spectra of the fully oxidized species, obtained by treating the protein with ferricyanide, show the simultaneous presence of peaks corresponding to different hexacoordinated states, the aquomet and the hemichrome. In order to assign the heme region state of the alpha and beta chains, the air-oxidized and ferricyanide-oxidized forms of Trematomus bernacchii hemoglobin were crystallized. Crystallographic analysis revealed that these forms correspond to an alpha(aquomet)-beta(bishistidyl-hemichrome) state. This demonstrates that the alpha and beta chains of Antarctic fish hemoglobins follow very different oxidation pathways. As found for Trematomus newnesi hemoglobin in a partial hemichrome state [Riccio, A., Vitagliano, L., di Prisco, G., Zagari, A. &amp; Mazzarella, L. (2002) Proc. Natl Acad. Sci. USA99, 9801-9806], the quaternary structures of these alpha(aquomet)-beta(bishistidyl-hemichrome) forms are intermediate between the physiological R and T hemoglobin states. Together, these structures provide information on the general features of this intermediate state.
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[[Category: Large Structures]]
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==About this Structure==
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1S5Y is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Trematomus_bernacchii Trematomus bernacchii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S5Y OCA].
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==Reference==
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The oxidation process of Antarctic fish hemoglobins., Vitagliano L, Bonomi G, Riccio A, di Prisco G, Smulevich G, Mazzarella L, Eur J Biochem. 2004 May;271(9):1651-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15096204 15096204]
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[[Category: Protein complex]]
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[[Category: Trematomus bernacchii]]
[[Category: Trematomus bernacchii]]
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[[Category: Bonomi, G.]]
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[[Category: Bonomi G]]
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[[Category: Mazzarella, L.]]
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[[Category: Mazzarella L]]
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[[Category: Prisco, G di.]]
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[[Category: Riccio A]]
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[[Category: Riccio, A.]]
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[[Category: Smulevich G]]
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[[Category: Smulevich, G.]]
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[[Category: Vitagliano L]]
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[[Category: Vitagliano, L.]]
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[[Category: Di Prisco G]]
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[[Category: bis-histidine]]
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[[Category: hemichrome]]
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[[Category: oxidation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:37:53 2008''
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Current revision

The crystal structure of Trematomus bernacchii hemoglobin oxidized by ferricyanide

PDB ID 1s5y

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