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1s6r

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[[Image:1s6r.gif|left|200px]]
 
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{{Structure
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==908R class c beta-lactamase bound to iodo-acetamido-phenyl boronic acid==
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|PDB= 1s6r |SIZE=350|CAPTION= <scene name='initialview01'>1s6r</scene>, resolution 2.24&Aring;
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<StructureSection load='1s6r' size='340' side='right'caption='[[1s6r]], [[Resolution|resolution]] 2.24&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=IAP:4-IODO-ACETAMIDO PHENYLBORONIC ACID'>IAP</scene>
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<table><tr><td colspan='2'>[[1s6r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S6R FirstGlance]. <br>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6. 3.5.2.6.]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.24&#8491;</td></tr>
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|GENE=
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IAP:4-IODO-ACETAMIDO+PHENYLBORONIC+ACID'>IAP</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s6r OCA], [https://pdbe.org/1s6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s6r RCSB], [https://www.ebi.ac.uk/pdbsum/1s6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s6r ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q93CA2_ENTCL Q93CA2_ENTCL]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s6/1s6r_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s6r ConSurf].
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<div style="clear:both"></div>
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'''908R CLASS C BETA-LACTAMASE BOUND TO IODO-ACETAMIDO-PHENYL BORONIC ACID'''
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==See Also==
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*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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The structures of the class C beta-lactamase from Enterobacter cloacae 908R alone and in complex with a boronic acid transition-state analogue were determined by X-ray crystallography at 2.1 and 2.3 A, respectively. The structure of the enzyme resembles those of other class C beta-lactamases. The structure of the complex with the transition-state analogue, iodo-acetamido-phenyl boronic acid, shows that the inhibitor is covalently bound to the active-site serine (Ser64). Binding of the inhibitor within the active site is compared with previously determined structures of complexes with other class C enzymes. The structure of the boronic acid adduct indicates ways to improve the affinity of this class of inhibitors. This structure of 908R class C beta-lactamase in complex with a transition-state analogue provides further insights into the mechanism of action of these hydrolases.
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==About this Structure==
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1S6R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S6R OCA].
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==Reference==
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Crystal structure of Enterobacter cloacae 908R class C beta-lactamase bound to iodo-acetamido-phenyl boronic acid, a transition-state analogue., Wouters J, Fonze E, Vermeire M, Frere JM, Charlier P, Cell Mol Life Sci. 2003 Aug;60(8):1764-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14521155 14521155]
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[[Category: Enterobacter cloacae]]
[[Category: Enterobacter cloacae]]
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[[Category: Hydrolase]]
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[[Category: Large Structures]]
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[[Category: Single protein]]
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[[Category: Wouters J]]
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[[Category: Wouters, J.]]
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[[Category: IAP]]
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[[Category: hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:01:11 2008''
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Current revision

908R class c beta-lactamase bound to iodo-acetamido-phenyl boronic acid

PDB ID 1s6r

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