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1s9r

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[[Image:1s9r.gif|left|200px]]
 
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==CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE==
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The line below this paragraph, containing "STRUCTURE_1s9r", creates the "Structure Box" on the page.
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<StructureSection load='1s9r' size='340' side='right'caption='[[1s9r]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1s9r]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycoplasmopsis_arginini Mycoplasmopsis arginini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S9R FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ARG:ARGININE'>ARG</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
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{{STRUCTURE_1s9r| PDB=1s9r | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s9r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s9r OCA], [https://pdbe.org/1s9r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s9r RCSB], [https://www.ebi.ac.uk/pdbsum/1s9r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s9r ProSAT]</span></td></tr>
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</table>
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'''CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE'''
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== Function ==
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[https://www.uniprot.org/uniprot/ARCA_MYCAR ARCA_MYCAR]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Arginine deiminase (ADI), an enzyme that hydrolyzes arginine to generate energy in many parasitic microorganisms, has potent anticancer activities and can halt growth of solid tumors. We determined the crystal structure of ADI from Mycoplasma arginini in two different forms (1.6 and 2.0 A resolution) using multiple isomorphous replacement. ADI shares common structural features with the arginine-catabolizing enzymes Arg:Gly amidinotransferase and dimethylarginine dimethyl-aminohydrolase; ADI contains an additional domain of five helices. The scissile C-N bonds of the substrates and the catalytic triads (Cys398-His269-Glu213 of ADI) for the three enzymes superimpose on each other. The ADI structure from form I crystals corresponds to a tetrahedral intermediate with four heteroatoms (1S, 2N, 1O) covalently bonded to the reaction-center carbon. The structure from form II crystals represents an amidino-enzyme complex; the reaction-center carbon is covalently bonded to Cys398 sulfur and two nitrogens, and the reacting water molecule is only 2.54 A away.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s9/1s9r_consurf.spt"</scriptWhenChecked>
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1S9R is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycoplasma_arginini Mycoplasma arginini]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9R OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Crystal structures of arginine deiminase with covalent reaction intermediates; implications for catalytic mechanism., Das K, Butler GH, Kwiatkowski V, Clark AD Jr, Yadav P, Arnold E, Structure. 2004 Apr;12(4):657-67. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15062088 15062088]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s9r ConSurf].
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[[Category: Arginine deiminase]]
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<div style="clear:both"></div>
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[[Category: Mycoplasma arginini]]
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__TOC__
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[[Category: Single protein]]
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</StructureSection>
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[[Category: Arnold, E.]]
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[[Category: Large Structures]]
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[[Category: Buttler, G H.]]
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[[Category: Mycoplasmopsis arginini]]
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[[Category: Das, K.]]
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[[Category: Arnold E]]
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[[Category: Jr., A D.Clark.]]
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[[Category: Buttler GH]]
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[[Category: Kwiatkowski, V.]]
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[[Category: Clark Jr AD]]
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[[Category: Yadav, P.]]
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[[Category: Das K]]
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[[Category: 5-fold pseudo-symmetric domain]]
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[[Category: Kwiatkowski V]]
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[[Category: 5-helix bundle domain]]
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[[Category: Yadav P]]
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[[Category: Deiminase]]
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[[Category: Hydrolase]]
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[[Category: Raction intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:27:41 2008''
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Current revision

CRYSTAL STRUCTURE OF ARGININE DEIMINASE COVALENTLY LINKED WITH A REACTION INTERMEDIATE

PDB ID 1s9r

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