1saz

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (08:30, 1 May 2024) (edit) (undo)
 
(13 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1saz.gif|left|200px]]
 
-
<!--
+
==Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima==
-
The line below this paragraph, containing "STRUCTURE_1saz", creates the "Structure Box" on the page.
+
<StructureSection load='1saz' size='340' side='right'caption='[[1saz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1saz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SAZ FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
-->
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACP:PHOSPHOMETHYLPHOSPHONIC+ACID+ADENYLATE+ESTER'>ACP</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-
{{STRUCTURE_1saz| PDB=1saz | SCENE= }}
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1saz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1saz OCA], [https://pdbe.org/1saz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1saz RCSB], [https://www.ebi.ac.uk/pdbsum/1saz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1saz ProSAT]</span></td></tr>
-
 
+
</table>
-
'''Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima'''
+
== Function ==
-
 
+
[https://www.uniprot.org/uniprot/BUK2_THEMA BUK2_THEMA]
-
 
+
== Evolutionary Conservation ==
-
==Overview==
+
[[Image:Consurf_key_small.gif|200px|right]]
-
The sitting-drop method of crystallization uses the evaporation of water to increase the concentration of the protein and precipitant in the drop. The presence of other volatile components, such as acetic acid, can have a marked impact on crystallization. A member of the ASKHA (acetate and sugar kinases/Hsc70/actin) superfamily of proteins, isobutyrate kinase (Buk2) from Thermotoga maritima, was expressed in Escherichia coli with six histidine residues added to the C-terminus. The purified protein was crystallized in a sitting drop with a well solution consisting of 1.7-3.0 M sodium formate, with the pH of the well solution alone adjusted to 4.5 with acetic acid. Diffraction data collected at 100 K show that the crystals diffract to 3.1 A and belong to space group I422, with unit-cell parameters a = b = 198.12, c = 58.93 A. Both the crystal form and the results of dynamic light-scattering studies suggest that Buk2 is an octomer, the first to be identified in the ASKHA superfamily.
+
Check<jmol>
-
 
+
<jmolCheckbox>
-
==About this Structure==
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sa/1saz_consurf.spt"</scriptWhenChecked>
-
1SAZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SAZ OCA].
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
 
+
<text>to colour the structure by Evolutionary Conservation</text>
-
==Reference==
+
</jmolCheckbox>
-
Crystallization of butyrate kinase 2 from Thermotoga maritima mediated by vapor diffusion of acetic acid., Diao J, Cooper DR, Sanders DA, Hasson MS, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1100-2. Epub 2003, May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12777787 12777787]
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1saz ConSurf].
-
[[Category: Butyrate kinase]]
+
<div style="clear:both"></div>
-
[[Category: Single protein]]
+
__TOC__
 +
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Thermotoga maritima]]
[[Category: Thermotoga maritima]]
-
[[Category: Cooper, D R.]]
+
[[Category: Cooper DR]]
-
[[Category: Diao, J S.]]
+
[[Category: Diao J]]
-
[[Category: Hasson, M S.]]
+
[[Category: Hasson MS]]
-
[[Category: Sanders, D A.]]
+
[[Category: Sanders DA]]
-
[[Category: Acetate kinase]]
+
-
[[Category: Amppcp]]
+
-
[[Category: Butyrate]]
+
-
[[Category: Butyrate kinase]]
+
-
[[Category: Disulfide bond]]
+
-
[[Category: Enzyme mechanism]]
+
-
[[Category: Hsc70]]
+
-
[[Category: Isobutyrate]]
+
-
[[Category: Isobutyrate kinase]]
+
-
[[Category: Two similar domain]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 08:29:50 2008''
+

Current revision

Membership in the ASKHA Superfamily: Enzymological Properties and Crystal Structure of Butyrate Kinase 2 from Thermotoga maritima

PDB ID 1saz

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1saz"
Personal tools