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1sfw

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[[Image:1sfw.gif|left|200px]]<br />
 
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<applet load="1sfw" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1sfw" />
 
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'''PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, 18 STRUCTURES'''<br />
 
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==Overview==
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==PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, 18 STRUCTURES==
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The three-dimensional structure of porcine pancreatic PLA2 (PLA2), present, in a 40 kDa ternary complex with micelles and a competitive inhibitor, has, been determined using multidimensional heteronuclear NMR spectroscopy. The, structure of the protein (124 residues) is based on 1854 constraints, comprising 1792 distance and 62 phi torsion angle constraints. A total of, 18 structures was calculated using a combined approach of distance, geometry and restrained molecular dynamics. The atomic rms distribution, about the mean coordinate positions for residues 1-62 and 72-124 is 0.75, +/- 0.09 A for the backbone atoms and 1.14 +/- 0.10 A for all atoms. The, rms difference between the averaged minimized NMR structures of the free, PLA2 and PLA2 in the ternary complex is 3.5 A for the backbone ... [[http://ispc.weizmann.ac.il/pmbin/getpm?7703697 (full description)]]
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<StructureSection load='1sfw' size='340' side='right'caption='[[1sfw]]' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1sfw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SFW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SFW FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sfw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sfw OCA], [https://pdbe.org/1sfw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sfw RCSB], [https://www.ebi.ac.uk/pdbsum/1sfw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sfw ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PA21B_PIG PA21B_PIG] PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sf/1sfw_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sfw ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SFW is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]] with CA as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4]]. Structure known Active Site: CA. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SFW OCA]].
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*[[Phospholipase A2 3D structures|Phospholipase A2 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Solution structure of porcine pancreatic phospholipase A2 complexed with micelles and a competitive inhibitor., van den Berg B, Tessari M, Boelens R, Dijkman R, Kaptein R, de Haas GH, Verheij HM, J Biomol NMR. 1995 Feb;5(2):110-21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=7703697 7703697]
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[[Category: Large Structures]]
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[[Category: Phospholipase A(2)]]
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[[Category: Single protein]]
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[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Berg, B.Van.Den.]]
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[[Category: Boelens R]]
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[[Category: Boelens, R.]]
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[[Category: De Haas GH]]
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[[Category: Dijkman, R.]]
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[[Category: Dijkman R]]
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[[Category: Haas, G.H.De.]]
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[[Category: Kaptein R]]
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[[Category: Kaptein, R.]]
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[[Category: Tessari M]]
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[[Category: Tessari, M.]]
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[[Category: Van Den Berg B]]
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[[Category: Verheij, H.M.]]
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[[Category: Verheij HM]]
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[[Category: CA]]
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[[Category: phosphatide-2-acyl-hydrolase]]
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[[Category: phospholipase a2]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 13:51:04 2007''
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PORCINE PANCREAS PHOSPHOLIPASE A2, NMR, 18 STRUCTURES

PDB ID 1sfw

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