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1shk

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[[Image:1shk.gif|left|200px]]<br />
 
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<applet load="1shk" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1shk, resolution 1.9&Aring;" />
 
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'''THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI'''<br />
 
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==Overview==
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==THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI==
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Shikimate kinase from Erwinia chrysanthemi, overexpressed in Escherichia, coli has been crystallized by the vapour-diffusion method using sodium, chloride as a precipitant. Mass spectrometry was used to confirm the, purity of the shikimate kinase and dynamic light scattering was used to, assess conditions for the monodispersity of the enzyme. The crystals are, tetragonal, space group P4(1)2(1)2 or enantiomorph with cell dimensions a, = b = 108.5 and c = 92.8 A (at 100 K). Native crystals diffract to better, than 2.6 A on a synchrotron X-ray source. The asymmetric unit is likely to, contain two molecules, corresponding to a packing density of 3.6 A(3), Da(-1).
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<StructureSection load='1shk' size='340' side='right'caption='[[1shk]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1shk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_chrysanthemi Dickeya chrysanthemi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SHK FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1shk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1shk OCA], [https://pdbe.org/1shk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1shk RCSB], [https://www.ebi.ac.uk/pdbsum/1shk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1shk ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/AROL_DICCH AROL_DICCH] Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.<ref>PMID:11369852</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sh/1shk_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1shk ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SHK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Erwinia_chrysanthemi Erwinia chrysanthemi]] with MG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Transferase Transferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.71 2.7.1.71]]. Structure known Active Sites: PIP, POP, SAS and SBS. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SHK OCA]].
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*[[Shikimate kinase 3D structures|Shikimate kinase 3D structures]]
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== References ==
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==Reference==
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<references/>
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Crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Erwinia chrysanthemi., Krell T, Coyle JE, Horsburgh MJ, Coggins JR, Lapthorn AJ, Acta Crystallogr D Biol Crystallogr. 1997 Sep 1;53(Pt 5):612-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299895 15299895]
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__TOC__
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[[Category: Erwinia chrysanthemi]]
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</StructureSection>
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[[Category: Single protein]]
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[[Category: Dickeya chrysanthemi]]
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[[Category: Coggins, J.R.]]
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[[Category: Large Structures]]
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[[Category: Krell, T.]]
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[[Category: Coggins JR]]
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[[Category: Lapthorn, A.J.]]
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[[Category: Krell T]]
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[[Category: MG]]
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[[Category: Lapthorn AJ]]
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[[Category: adp]]
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[[Category: p-loop protein]]
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[[Category: phosphoryl transfer]]
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[[Category: shikimate kinase]]
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[[Category: shikimate pathway]]
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[[Category: transferase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 08:32:41 2007''
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Current revision

THE THREE-DIMENSIONAL STRUCTURE OF SHIKIMATE KINASE FROM ERWINIA CHRYSANTHEMI

PDB ID 1shk

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