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1ska

From Proteopedia

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(New page: 200px<br /><applet load="1ska" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ska, resolution 1.69&Aring;" /> '''Crystallographic sna...)
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[[Image:1ska.gif|left|200px]]<br /><applet load="1ska" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ska, resolution 1.69&Aring;" />
 
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'''Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics'''<br />
 
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==Overview==
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==Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics==
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Understanding of the atomic movements involved in an enzymatic reaction, needs structural information on the active and inactive native enzyme, molecules and on the enzyme-substrate, enzyme-intermediate, and, enzyme-product(s) complexes. By using the X-ray crystallographic method, four crystal structures of Aspergillus fumigatus phytase were obtained at, resolution higher than 1.7 A. The pH-dependent catalytic activity of A., fumigatus phytase was linked to three water molecules that may prevent the, substrate from binding and thus block nucleophilic attack of the catalytic, imidazole nitrogen. Comparison of various structures also identified the, water molecule that attacks the phosphamide bond during the hydrolysis, process, and established the hydrolysis pathway of the intermediate., Additionally, two reaction product phosphates were observed at the active, site, suggesting a possible product release pathway after hydrolysis of, the intermediate. These results can help explain the catalytic mechanism, throughout the whole acid phosphatase family, as all key residues are, conserved.
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<StructureSection load='1ska' size='340' side='right'caption='[[1ska]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ska]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SKA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SKA FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ska FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ska OCA], [https://pdbe.org/1ska PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ska RCSB], [https://www.ebi.ac.uk/pdbsum/1ska PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ska ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHYA_ASPFU PHYA_ASPFU] Catalyzes the hydrolysis of inorganic orthophosphate from phytate.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sk/1ska_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ska ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SKA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_fumigatus Aspergillus fumigatus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SKA OCA].
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*[[Phytase 3D structures|Phytase 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Crystallographic snapshots of Aspergillus fumigatus phytase, revealing its enzymatic dynamics., Liu Q, Huang Q, Lei XG, Hao Q, Structure. 2004 Sep;12(9):1575-83. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15341723 15341723]
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[[Category: 3-phytase]]
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[[Category: Aspergillus fumigatus]]
[[Category: Aspergillus fumigatus]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Hao, Q.]]
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[[Category: Hao Q]]
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[[Category: Huang, Q.]]
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[[Category: Huang Q]]
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[[Category: Lei, X.G.]]
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[[Category: Lei XG]]
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[[Category: Liu, Q.]]
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[[Category: Liu Q]]
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[[Category: NAG]]
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[[Category: big alpha/beta domain]]
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[[Category: catalytic dynamics]]
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[[Category: catalytic sites]]
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[[Category: product release pathway]]
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[[Category: small alpha domain]]
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[[Category: water structures]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:26:08 2007''
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Current revision

Crystallographic snapshots of Aspergillus fumigatus phytase revealing its enzymatic dynamics

PDB ID 1ska

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