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1smn

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(New page: 200px<br /><applet load="1smn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1smn, resolution 2.04&Aring;" /> '''IDENTIFICATION OF TH...)
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[[Image:1smn.gif|left|200px]]<br /><applet load="1smn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1smn, resolution 2.04&Aring;" />
 
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'''IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS'''<br />
 
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==Overview==
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==IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS==
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The Serratia endonuclease is an extracellularly secreted enzyme capable of, cleaving both single- and double-stranded forms of DNA and RNA. It is the, first member of a large class of related and usually dimeric endonucleases, for which a structure is known. Using X-ray crystallography, the structure, of monomer of this enzyme was reported by us previously (Miller MD et al., 1994, Nature Struct Biol 1:461-468). We now confirm the dimeric nature of, this enzyme through light-scattering experiments and identify the, physiologic dimer interface through crystal packing analysis. This, dimerization occurs through an isologous twofold interaction localized to, the carboxy-terminal subdomain of the enzyme. The dimer is a prolate, ellipsoid with dimensions 30 A x 35 A x 90 A. The dimer interface is flat, and contains four salt links, several hydrogen bonds, and nonpolar, interactions. Buried water is prominent in this interface and it includes, an unusual "cubic" water cluster. The position of the two active sites in, the dimer suggests that they can act independently in their cleavage of, DNA, but have a geometrical advantage in attacking substrate relative to, the monomer.
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<StructureSection load='1smn' size='340' side='right'caption='[[1smn]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1smn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SMN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SMN FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1smn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1smn OCA], [https://pdbe.org/1smn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1smn RCSB], [https://www.ebi.ac.uk/pdbsum/1smn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1smn ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/NUCA_SERMA NUCA_SERMA] Catalyzes the hydrolysis of both DNA and RNA, double- or single-stranded, at the 3'position of the phosphodiester bond to produce 5'-phosphorylated mono-, di-, tri- and tetranucleotides. DNA is a slightly better substrate than RNA.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sm/1smn_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1smn ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SMN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Serratia_marcescens Serratia marcescens]. Active as [http://en.wikipedia.org/wiki/Serratia_marcescens_nuclease Serratia marcescens nuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.30.2 3.1.30.2] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SMN OCA].
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*[[Endonuclease 3D structures|Endonuclease 3D structures]]
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__TOC__
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==Reference==
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</StructureSection>
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Identification of the Serratia endonuclease dimer: structural basis and implications for catalysis., Miller MD, Krause KL, Protein Sci. 1996 Jan;5(1):24-33. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8771193 8771193]
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[[Category: Large Structures]]
[[Category: Serratia marcescens]]
[[Category: Serratia marcescens]]
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[[Category: Serratia marcescens nuclease]]
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[[Category: Krause KL]]
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[[Category: Single protein]]
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[[Category: Miller MD]]
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[[Category: Krause, K.L.]]
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[[Category: Miller, M.D.]]
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[[Category: dnase]]
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[[Category: nuclease]]
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[[Category: rnase]]
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[[Category: sugar-nonspecific nuclease]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:29:35 2007''
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Current revision

IDENTIFICATION OF THE SERRATIA ENDONUCLEASE DIMER: STRUCTURAL BASIS AND IMPLICATIONS FOR CATALYSIS

PDB ID 1smn

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