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1ssb

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(New page: 200px<br /><applet load="1ssb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ssb, resolution 2.0&Aring;" /> '''A STRUCTURAL INVESTIG...)
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[[Image:1ssb.gif|left|200px]]<br /><applet load="1ssb" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ssb, resolution 2.0&Aring;" />
 
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'''A STRUCTURAL INVESTIGATION OF CATALYTICALLY MODIFIED F12OL AND F12OY SEMISYNTHETIC RIBONUCLEASES'''<br />
 
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==Overview==
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==A STRUCTURAL INVESTIGATION OF CATALYTICALLY MODIFIED F12OL AND F12OY SEMISYNTHETIC RIBONUCLEASES==
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The structures of two catalytically modified semisynthetic RNases obtained, by replacing phenylalanine 120 with leucine and tyrosine have been, determined and refined at a resolution of 2.0 A (R = 0.161 and 0.184, respectively). These structures have been compared with the refined 1.8-A, structure (R = 0.204) of the fully active phenylalanine-containing enzyme, (Martin PD, Doscher MS, Edwards BFP, 1987, J Biol Chem 262:15930-15938), and with the catalytically defective D121A (2.0 A, R = 0.172) and D121N, (2.0 A, R = 0.186) analogs (deMel VSJ, Martin PD, Doscher MS, Edwards BFP, 1992, J Biol Chem 267:247-256). The movement away from the active site of, the loop containing residues 65-72 is seen in all three catalytically, defective analogs--F120L, D121A, and D121N--but not in the fully active, (or hyperactive) F120Y. The insertion of the phenolic hydroxyl of Tyr 120, into a hydrogen-bonding network involving the hydroxyl group of Ser 123, and a water molecule in F120Y is the likely basis for the hyperactivity, toward uridine 2',3'-cyclic phosphate previously found for this analog, (Hodges RS, Merrifield RB, 1974, Int J Pept Protein Res 6:397-405) as well, as the threefold increase in KM for cytidine 2',3'-cyclic phosphate found, for this analog by ourselves.
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<StructureSection load='1ssb' size='340' side='right'caption='[[1ssb]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[1ssb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SSB FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ssb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ssb OCA], [https://pdbe.org/1ssb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ssb RCSB], [https://www.ebi.ac.uk/pdbsum/1ssb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ssb ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RNAS1_BOVIN RNAS1_BOVIN] Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA.<ref>PMID:7479688</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ss/1ssb_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ssb ConSurf].
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<div style="clear:both"></div>
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==About this Structure==
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==See Also==
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1SSB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SSB OCA].
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*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
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== References ==
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==Reference==
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<references/>
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Structural investigation of catalytically modified F120L and F120Y semisynthetic ribonucleases., deMel VS, Doscher MS, Glinn MA, Martin PD, Ram ML, Edwards BF, Protein Sci. 1994 Jan;3(1):39-50. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8142897 8142897]
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__TOC__
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[[Category: Protein complex]]
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</StructureSection>
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[[Category: Demel, V.S.J.]]
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[[Category: Bos taurus]]
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[[Category: Doscher, M.S.]]
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[[Category: Large Structures]]
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[[Category: Edwards, B.F.P.]]
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[[Category: Demel VSJ]]
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[[Category: Glinn, M.A.]]
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[[Category: Doscher MS]]
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[[Category: Martin, P.D.]]
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[[Category: Edwards BFP]]
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[[Category: Ram, M.L.]]
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[[Category: Glinn MA]]
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[[Category: SO4]]
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[[Category: Martin PD]]
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[[Category: hydrolase(endoribonuclease)]]
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[[Category: Ram ML]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 02:37:24 2007''
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Current revision

A STRUCTURAL INVESTIGATION OF CATALYTICALLY MODIFIED F12OL AND F12OY SEMISYNTHETIC RIBONUCLEASES

PDB ID 1ssb

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