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1ssu

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(New page: 200px<br /> <applet load="1ssu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ssu" /> '''Structural and biochemical evidence for dis...)
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[[Image:1ssu.gif|left|200px]]<br />
 
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<applet load="1ssu" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1ssu" />
 
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'''Structural and biochemical evidence for disulfide bond heterogeneity in active forms of the somatomedin B domain of human vitronectin'''<br />
 
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==Overview==
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==Structural and biochemical evidence for disulfide bond heterogeneity in active forms of the somatomedin B domain of human vitronectin==
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The N-terminal cysteine-rich somatomedin B (SMB) domain (residues 1-44) of, the human glycoprotein vitronectin contains the high-affinity binding, sites for plasminogen activator inhibitor-1 (PAI-1) and the urokinase, receptor (uPAR). We previously showed that the eight cysteine residues of, recombinant SMB (rSMB) are organized into four disulfide bonds in a linear, uncrossed pattern (Cys(5)-Cys(9), Cys(19)-Cys(21), Cys(25)-Cys(31), and, Cys(32)-Cys(39)). In the present study, we use an alternative method to, show that this disulfide bond arrangement remains a major preferred one in, solution, and we determine the solution structure of the domain using NMR, analysis. The solution structure shows that the four disulfide bonds are, tightly packed in the center of the domain, replacing the traditional, hydrophobic core expected for a globular protein. The few noncysteine, hydrophobic side chains form a cluster on the outside of the domain, providing a distinctive binding surface for the physiological partners, PAI-1 and uPAR. The hydrophobic surface consists mainly of side chains, from the loop formed by the Cys(25)-Cys(31) disulfide bond, and is, surrounded by conserved acidic and basic side chains, which are likely to, contribute to the specificity of the intermolecular interactions of this, domain. Interestingly, the overall fold of the molecule is compatible with, several arrangements of the disulfide bonds. A number of different, disulfide bond arrangements were able to satisfy the NMR restraints, and, an extensive series of conformational energy calculations performed in, explicit solvent confirmed that several disulfide bond arrangements have, comparable stabilization energies. An experimental demonstration of the, presence of alternative disulfide conformations in active rSMB is provided, by the behavior of a mutant in which Asn(14) is replaced by Met. This, mutant has the same PAI-1 binding activity as rVN1-51, but its, fragmentation pattern following cyanogen bromide treatment is incompatible, with the linear uncrossed disulfide arrangement. These results suggest, that active forms of the SMB domain may have a number of allowed disulfide, bond arrangements as long as the Cys(25)-Cys(31) disulfide bond is, preserved.
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<StructureSection load='1ssu' size='340' side='right'caption='[[1ssu]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1ssu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SSU FirstGlance]. <br>
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1SSU is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1SSU OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ssu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ssu OCA], [https://pdbe.org/1ssu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ssu RCSB], [https://www.ebi.ac.uk/pdbsum/1ssu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ssu ProSAT]</span></td></tr>
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==Reference==
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</table>
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Disulfide bonding arrangements in active forms of the somatomedin B domain of human vitronectin., Kamikubo Y, De Guzman R, Kroon G, Curriden S, Neels JG, Churchill MJ, Dawson P, Oldziej S, Jagielska A, Scheraga HA, Loskutoff DJ, Dyson HJ, Biochemistry. 2004 Jun 1;43(21):6519-34. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15157085 15157085]
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== Function ==
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[https://www.uniprot.org/uniprot/VTNC_HUMAN VTNC_HUMAN] Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ss/1ssu_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ssu ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Churchill, M.J.]]
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[[Category: Churchill MJ]]
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[[Category: Curriden, S.]]
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[[Category: Curriden S]]
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[[Category: Dawson, P.]]
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[[Category: Dawson P]]
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[[Category: Dyson, H.J.]]
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[[Category: De Guzman R]]
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[[Category: Guzman, R.De.]]
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[[Category: Dyson HJ]]
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[[Category: Jagielska, A.]]
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[[Category: Jagielska A]]
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[[Category: Kamikubo, Y.]]
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[[Category: Kamikubo Y]]
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[[Category: Kroon, G.]]
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[[Category: Kroon G]]
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[[Category: Loskutoff, D.J.]]
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[[Category: Loskutoff DJ]]
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[[Category: Neels, J.G.]]
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[[Category: Neels JG]]
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[[Category: Oldziej, S.]]
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[[Category: Oldziej S]]
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[[Category: Scheraga, H.A.]]
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[[Category: Scheraga HA]]
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[[Category: disulfide bonds heterogeneity]]
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[[Category: nmr structure]]
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[[Category: somatostatin b domain]]
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[[Category: vitronectin]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 19:17:28 2007''
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Current revision

Structural and biochemical evidence for disulfide bond heterogeneity in active forms of the somatomedin B domain of human vitronectin

PDB ID 1ssu

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