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1sw1

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[[Image:1sw1.gif|left|200px]]
 
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==Crystal structure of ProX from Archeoglobus fulgidus in complex with proline betaine==
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The line below this paragraph, containing "STRUCTURE_1sw1", creates the "Structure Box" on the page.
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<StructureSection load='1sw1' size='340' side='right'caption='[[1sw1]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1sw1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_DSM_4304 Archaeoglobus fulgidus DSM 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SW1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SW1 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PBE:1,1-DIMETHYL-PROLINIUM'>PBE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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{{STRUCTURE_1sw1| PDB=1sw1 | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sw1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sw1 OCA], [https://pdbe.org/1sw1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sw1 RCSB], [https://www.ebi.ac.uk/pdbsum/1sw1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sw1 ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of ProX from Archeoglobus fulgidus in complex with proline betaine'''
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== Function ==
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[https://www.uniprot.org/uniprot/O29280_ARCFU O29280_ARCFU]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Compatible solutes such as glycine betaine and proline betaine serve as protein stabilizers because of their preferential exclusion from protein surfaces. To use extracellular sources of this class of compounds as osmo-, cryo-, or thermoprotectants, Bacteria and Archaea have developed high affinity uptake systems of the ATP-binding cassette type. These transport systems require periplasmic- or extracellular-binding proteins that are able to bind the transported substance with high affinity. Therefore, binding proteins that bind compatible solutes have to avoid the exclusion of their ligands within the binding pocket. In the present study we addressed the question to how compatible solutes can be effectively bound by a protein at temperatures around 83 degrees C as this is done by the ligand-binding protein ProX from the hyperthermophilic archaeon Archaeoglobus fulgidus. We solved the structures of ProX without ligand and in complex with both of its natural ligands glycine betaine and proline betaine, as well as in complex with the artificial ligand trimethylammonium. Cation-pi interactions and non-classical hydrogen bonds between four tyrosine residues, a main chain carbonyl oxygen, and the ligand have been identified to be the key determinants in binding the quaternary amines of the three investigated ligands. The comparison of the ligand binding sites of ProX from A. fulgidus and the recently solved structure of ProX from Escherichia coli revealed a very similar solution for the problem of compatible solute binding, although both proteins share only a low degree of sequence identity. The residues involved in ligand binding are functionally equivalent but not conserved in the primary sequence.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sw/1sw1_consurf.spt"</scriptWhenChecked>
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1SW1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus_dsm_4304 Archaeoglobus fulgidus dsm 4304]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SW1 OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structural basis for the binding of compatible solutes by ProX from the hyperthermophilic archaeon Archaeoglobus fulgidus., Schiefner A, Holtmann G, Diederichs K, Welte W, Bremer E, J Biol Chem. 2004 Nov 12;279(46):48270-81. Epub 2004 Aug 11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15308642 15308642]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sw1 ConSurf].
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[[Category: Archaeoglobus fulgidus dsm 4304]]
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<div style="clear:both"></div>
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[[Category: Single protein]]
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__TOC__
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[[Category: Bremer, E.]]
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</StructureSection>
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[[Category: Diederichs, K.]]
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[[Category: Archaeoglobus fulgidus DSM 4304]]
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[[Category: Holtmann, G.]]
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[[Category: Large Structures]]
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[[Category: Schiefner, A.]]
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[[Category: Bremer E]]
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[[Category: Welte, W.]]
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[[Category: Diederichs K]]
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[[Category: Binding-protein]]
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[[Category: Holtmann G]]
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[[Category: Cation-pi interaction]]
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[[Category: Schiefner A]]
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[[Category: Compatible solute]]
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[[Category: Welte W]]
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[[Category: Non-classical hydrogen bond]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:12:13 2008''
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Current revision

Crystal structure of ProX from Archeoglobus fulgidus in complex with proline betaine

PDB ID 1sw1

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